Bioinformatics Analysis of the Carotenoid Isomerase Gene in Cabbage (Brassica oleracea var. capitata)

DOI

10.2991/mseee-18.2018.49How to use a DOI?

Keywords

Carotenoid isomerase; gene sequences; bioinformatics.

Abstract

Carotenoid isomerase (CRTISO) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. capitata CRTISO (BocCRTISO) gene sequences were obtained from Brassica database (BRAD), and preformed for bioinformatics analysis. The BocCRTISO gene mapped to Scaffold000330, and contains an open reading frame of 1,773 bp that encodes a 590-amino acid protein with a calculated molecular mass of 64.99 kD and an isoelectric point (pI) of 6.75. Subcellular localization predicted the BocCRTISO gene was in the chloroplast. The conserved domain of the BocCRTISO protein is Rossmann-fold NAD (P)H/NAD(P)(+) binding (NADB) domain. Homology analysis indicates that the CRTISO protein is apparently conserved during plant evolution and is most closely related to B. napus, and B. rapa. The findings of the present study provide a molecular basis for the elucidation of CRTISO gene function in cabbage.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Bo Sun
AU  - Min Jiang
AU  - Fen Zhang
AU  - Haoru Tang
PY  - 2018/08
DA  - 2018/08
TI  - Bioinformatics Analysis of the Carotenoid Isomerase Gene in Cabbage (Brassica oleracea var. capitata)
BT  - Proceedings of the 2nd International Conference on Material Science, Energy and Environmental Engineering (MSEEE 2018)
PB  - Atlantis Press
SP  - 274
EP  - 279
SN  - 2352-5401
UR  - https://doi.org/10.2991/mseee-18.2018.49
DO  - 10.2991/mseee-18.2018.49
ID  - Sun2018/08
ER  -