Artery Research

Volume 25, Issue Supplement 1, December 2019, Pages S124 - S124

P81 Antibody to Cardiotonic Steroid Reduces Blood Pressure and Vascular Fibrosis in Preeclampsia

Authors
NI Agalakova3, VA Reznik1, OV Nadei3, IA Ershov1, OS Rassokha1, ML Vasyutina2, MM Galagudza2, AY Bagrov1, 3, *
1Department of Obstetrics and Gynecology, School of Pediatric Medicine
2Institute of Experimental Medicine, Almazov National Medical Research Centre
3Sechenov Institute of Evolutionary Physiology and Biochemistry, St. Petersburg, Russia
*Corresponding author. Email: aybagrov@gmail.com
Corresponding Author
AY Bagrov
Available Online 17 February 2020.
DOI
10.2991/artres.k.191224.111How to use a DOI?
Abstract

Background: Previous studies implicated cardiotonic steroids, including Na/K-ATPase inhibitor marinobufagenin (MBG), in the pathogenesis of preeclampsia (PE). We demonstrated that MBG induces fibrosis via mechanism involving inhibition of Fli1, a nuclear transcription factor and a negative regulator of collagen-1 synthesis.

Objectives and Methods: We hypothesized that PE blockade of increased MBG with antibody would lessen the fibrosis of umbilical arteries and lower the blood pressure in rats with PE. We tested 36 pregnant Sprague-Dawley rats in which 12 were made hypertensive by 1.8% Na supplementation (days 6–19 of gestation), 12 pregnant rats served controls. At day 19, PE rats received one intraperitoneal injection of polyclonal anti-MBG-4 antibody (0.5 ug/mL) for 4 hours.

Results: PE was associated with higher blood pressure (117 ± 2 vs 107 ± 2 mmHg; p < 0.01), plasma MBG levels (1.54 ± 0.34 vs 0.49 ± 0.11 nmol/L; p < 0.01), protein excretion (26 vs 12 mg/24 hours), sFlt-1 (4-fold), decrease in Fli1 (7-fold) and increase in collagen-1 in aorta (4-fold) vs. control rats (all p < 0.01). In 12 rats was treated with polyclonal anti-MBG-4 antibody blood pressure dropped (93 ± 3 mmHg) and Fli1 was decreased much less (2-fold; p < 0.01 vs nontreated rats).

Conclusion: These results demonstrate that in experimental PE elevated MBG level is implicated in umbilical fibrosis via suppression of Fli1. Supported by Russian Scientific Foundation grant № 18-15-00222.

Copyright
© 2019 Association for Research into Arterial Structure and Physiology. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Journal
Artery Research
Volume-Issue
25 - Supplement 1
Pages
S124 - S124
Publication Date
2020/02/17
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.2991/artres.k.191224.111How to use a DOI?
Copyright
© 2019 Association for Research into Arterial Structure and Physiology. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - JOUR
AU  - NI Agalakova
AU  - VA Reznik
AU  - OV Nadei
AU  - IA Ershov
AU  - OS Rassokha
AU  - ML Vasyutina
AU  - MM Galagudza
AU  - AY Bagrov
PY  - 2020
DA  - 2020/02/17
TI  - P81 Antibody to Cardiotonic Steroid Reduces Blood Pressure and Vascular Fibrosis in Preeclampsia
JO  - Artery Research
SP  - S124
EP  - S124
VL  - 25
IS  - Supplement 1
SN  - 1876-4401
UR  - https://doi.org/10.2991/artres.k.191224.111
DO  - 10.2991/artres.k.191224.111
ID  - Agalakova2020
ER  -