Artery Research

Volume 25, Issue Supplement 1, December 2019, Pages S192 - S192

P154 Effect of Vitamin D Deficiency on Insulin Induced Vasodilatation and Receptor Expression in Rat Model of Polycystic Ovary Syndrome

Authors
Bálint Bányai1, Benko Rita2, Tarszabó Róbert2, Lajtai Krisztina2, Horváth Eszter Mária2, Várbíró Szabolcs2
1Semmelweis University Department of Physiology, Budapest, Hungary
2Semmelweis University Department of Obstetrics and Gynaecology, Budapest, Hungary
Available Online 17 February 2020.
DOI
10.2991/artres.k.191224.174How to use a DOI?
Abstract

In polycystic ovary syndrome (PCOS) hyperandrogenism and metabolic dysfunction increase cardiovascular risk. Vitamin D3 deficiency is a common comorbidity in PCOS. Our aim was to examine the alterations of insulin-induced vasodilation and receptor expression in rat aorta in a PCOS model.

Methods: Female Wistar rats were treated as follows: 1. vitamin D supplemented group (D+T−); 2. vitamin D deficient (D−T−), 3. vitamin D supplemented with transdermal testosterone application (D+T+) and 4. vitamin D deficient with transdermal testosterone (D−T+). Wire myograph was used for testing insulin relaxation of aorta rings in physiological salt solution and under NOS inhibition. Insulin (IR) and vitamin D receptor (VDR) density was examined by immunohistochemistry.

Results: Insulin-induced vasodilatation of the aorta rings were significantly lower in both vitamin deficient compared to the vitamin supplemented groups (p < 0.05). NOS inhibition significantly reduce the relaxation. Aorta endothelial IR expression was significantly higher in the vitamin D deficient group, meanwhile in the testosterone-treated groups (D+T+; D−T+) the expression was significantly lower (Area%: D+: 0.830 ± 0.10; D+T+: 0.298 ± 0.06; D−: 1.364 ± 0.12; D−T+: 0.354 ± 0.15, p < 0.05 in D− & D+T+ & D−T+ vs D+. p < 0.01 D+T+ & D−T+ vs D−). VDR density was significantly higher in the vitamin D deficient groups in comparison to the supplemented groups (Area% VDR: D+: 41.56 ± 5.58 vs D−: 60.63 ± 5.23) Testosterone treatment have not any effect on VDR expression.

Conclusion: Vitamin-D deficiency causes impaired insulin induced vasodilation. Increased IR density could not compensate altered insulin-induced relaxation.

Copyright
© 2019 Association for Research into Arterial Structure and Physiology. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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Journal
Artery Research
Volume-Issue
25 - Supplement 1
Pages
S192 - S192
Publication Date
2020/02/17
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.2991/artres.k.191224.174How to use a DOI?
Copyright
© 2019 Association for Research into Arterial Structure and Physiology. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - JOUR
AU  - Bálint Bányai
AU  - Benko Rita
AU  - Tarszabó Róbert
AU  - Lajtai Krisztina
AU  - Horváth Eszter Mária
AU  - Várbíró Szabolcs
PY  - 2020
DA  - 2020/02/17
TI  - P154 Effect of Vitamin D Deficiency on Insulin Induced Vasodilatation and Receptor Expression in Rat Model of Polycystic Ovary Syndrome
JO  - Artery Research
SP  - S192
EP  - S192
VL  - 25
IS  - Supplement 1
SN  - 1876-4401
UR  - https://doi.org/10.2991/artres.k.191224.174
DO  - 10.2991/artres.k.191224.174
ID  - Bányai2020
ER  -