P.34 Preeclampsia Leads to the Delayed Development of Sympathetic Control of the Cardiovascular System in the Offspring
- 10.2991/artres.k.201209.047How to use a DOI?
- preeclampsia; hypertension; intrauterine programming
Background: Preeclampsia is a common pregnancy disease characterized by hypertension and kidney failure. Recent studies have shown that even in the case of successful delivery, preeclampsia could induce long-term effects in the offspring. Nevertheless, the effects of preeclampsia on the cardiovascular system of the offspring are poorly studied.
Methods: We induced preeclampsia in pregnant rats by L-NAME (nitric oxide synthase inhibitor) supplementation (250 mg/l in drinking water from gestation day 10 to delivery, daily dose 39 mg/kg). The model was verified by the dam’s blood pressure (BP) elevation (tail-cuff) and creatinine clearance reduction (metabolic cages) compared to control dams. Male offspring 16-18-day old were used for BP recording (catheter technique under urethane anesthesia); their isolated arteries were studied by wire myography. Adrenergic nerve plexus was visualized by glyoxylic acid staining.
Results: Offspring of dams with preeclampsia had reduced body weight compared to control. They also demonstrated decreased BP (43.3 ± 1.9 vs 50.5 ± 1.4 mmHg) and diminished response to the ganglionic blocker chlorizondamine (2.5 mg/kg); heart rate didn’t differ between the groups. The density of the sympathetic innervation of the right atrium and the saphenous artery was reduced in the preeclampsia offspring. Saphenous arteries from preeclampsia offspring had smaller diameter (276 ± 5 vs. 296 ± 7 micron) and maximal contraction force (9.4 ± 0.4 vs. 10.6 ± 0.4 mN) compared to control.
Conclusions: Preeclampsia is followed by the delay in sympathetic nervous system development in the offspring, which is accompanied by structural and functional alterations in the cardiovascular system.
The research was supported by Russian Science Foundation (Grant N 19-15-00210).
- © 2020 Association for Research into Arterial Structure and Physiology. Publishing services by Atlantis Press International B.V.
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- This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - JOUR AU - Ekaterina Selivanova AU - Anastasia Shvetsova AU - Victoria Potekhina AU - Dina Gaynullina AU - Anna Borzykh AU - Oxana Kiryukhina AU - Vladislav Kuzmin AU - Olga Tarasova PY - 2020 DA - 2020/12/31 TI - P.34 Preeclampsia Leads to the Delayed Development of Sympathetic Control of the Cardiovascular System in the Offspring JO - Artery Research SP - S57 EP - S57 VL - 26 IS - Supplement 1 SN - 1876-4401 UR - https://doi.org/10.2991/artres.k.201209.047 DO - 10.2991/artres.k.201209.047 ID - Selivanova2020 ER -