Second-line Drug Resistance Characterization in Mycobacterium tuberculosis by Genotype MTBDRsl Assay
First two authors are joint first author.
- https://doi.org/10.2991/jegh.k.191215.003How to use a DOI?
- Drug resistance, diagnosis, MTBDRsl Ver 2.0, Mycobacterium tuberculosis
Tuberculosis (TB) remains a main hurdle for national programs due to increase in drug resistance to antitubercular drugs. World Health Organization (WHO)-endorsed Line Probe Assay, Genotype MTBDRsl Ver 2.0, gives opportunity for rapid diagnosis and molecular characterization of different mutations in drug targets of fluoroquinolone (FQ) and second-line injectable drugs (SLID). We, retrospectively, analyzed the data of Genotype MTBDRsl Ver 2.0 from January 2018 to June 2018. A total of 863 isolates of Mycobacterium tuberculosis, 687 rifampicin resistant and 176 isoniazid resistant only, were screened for drug resistance in FQ and SLID. All the isolates were tested for Genotype MTBDRsl Ver 2.0 according to the manufacturer’s instructions. The FQ and SLID resistance were detected in 295 (34.2%) and 70 (8.1%) isolates, respectively. Among newly diagnosed and follow-up rifampicin-resistant TB (RR TB) patients, the FQ resistance was 25.8% and 44.5%, respectively. The most common mutation (42.7%) in FQ-resistant isolates was MUT3C in gyrA gene. Both SLID and FQ resistance were detected in 59 (6.8%) RR TB isolates. The mono SLID resistance was detected in 12 (1.7%) isolates of RR TB. Genotype MTBDRsl Ver 2.0 assay is a rapid and important tool for the diagnosis and molecular characterization of second-line drug resistance under programmatic conditions.
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- This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - JOUR AU - Sunil Sethi AU - Priyanka Agarwal AU - Rajiv Khaneja AU - Naresh Kumar AU - Nitin Kumar AU - Jagesh Chandna AU - Ashutosh Nath Aggarwal AU - Rakesh Yadav PY - 2020 DA - 2020/01 TI - Second-line Drug Resistance Characterization in Mycobacterium tuberculosis by Genotype MTBDRsl Assay JO - Journal of Epidemiology and Global Health SP - 42 EP - 45 VL - 10 IS - 1 SN - 2210-6014 UR - https://doi.org/10.2991/jegh.k.191215.003 DO - https://doi.org/10.2991/jegh.k.191215.003 ID - Sethi2020 ER -