The Effect and Mechanism of Apoptosis on Hela Cells induced by Artesunate

DOI

10.2991/aeece-15.2015.176How to use a DOI?

Keywords

mechanism, Apoptosis, Artesunate, Hela.

Abstract

Cytotoxic activity of Artesunate(ART) was determined by MTT assay at various concentrations ranging from 0 to 100 mol/L on Hela cells. The apoptosis effect of Hela cells treated with ART was observed by a phase contrast microscope. The Apoptosis and its mechanism induced by ART were also studied by real-time PCR (RT-PCR) and Western blot. The results showed that ART has significant proliferation inhibition effect to Hela cells and the IC50 value is 35.4 mol/L for 24 h. The expression of Bcl-2 decreased and the expression of Caspas-9 and Bid increased in the Hela cells treated with ART at concentrations from 0 to 100 mol/L. These data suggest that the apoptotic effect of ART is mediated through mitochondrial pathway in Hela cells.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Guoquan Wang
AU  - Xu Chao
AU  - Sha Li
AU  - Jin Yan
AU  - Zongmiao Hu
AU  - Chenguang Liang
AU  - Linzhong Yu
AU  - Enhu Zhang
PY  - 2015/09
DA  - 2015/09
TI  - The Effect and Mechanism of Apoptosis on Hela Cells induced by Artesunate
BT  - Proceedings of the International Conference on Advances in Energy, Environment and Chemical Engineering
PB  - Atlantis Press
SP  - 855
EP  - 859
SN  - 2352-5401
UR  - https://doi.org/10.2991/aeece-15.2015.176
DO  - 10.2991/aeece-15.2015.176
ID  - Wang2015/09
ER  -