Component composition of oat prolamins in A. strigosa Schreb., A. abyssinica Hochst., A. byzantina C. Koch. and A. sativa L.

DOI

10.2991/agrosmart-18.2018.90How to use a DOI?

Keywords

cultivated oat species, A. strigosa Schreb., A. abyssinica Hochst., A. sativa L., A. byzantina C. Koch, avenin, electrophoretic spectrum

Abstract

Modern selection and seed development widely use biotechnology methods for creation of new intense cultivars and subsequent monitoring of biotypical composition. Among those methods is using storage proteins (prolamins) for analysis of genetic diversity. Prolamins and avenins are used for assessment of genetic diversity of oat, however, a lack of a common approach to interpretation and registering of avenin spectra impedes application of available data on component composition of these proteins when assessing the oat-related source material. The aim of the research was to analyze the component composition of avenin in oat cultivars of various ploidity for subsequent use in marker selection when assessing the oat source material. As a result of the research, 173 protein components were identified, 65.8 % of them are species-specific. It has been established, that cultivated oat species positively differ from each other by the mean number of protein components in the spectra of the samples. The highest average number of the avenin protein components has been found in the common oat spectra, at 8.5±0.05. The lowest average number of the avenin protein components has been found for the lopsided oat and the Ethiopian oat at - 4.2±0.32 and 4.6±0.47 respectively. The maximum number of various avenin components has been discovered in the common oat spectra, 160 units, while for the Ethiopian oat there were only 7 units identified. This is an evidence of a high level of genetic diversity in the studied collection of A. sativa and a high probability to identify a large number of selection-valuable genotypes when conducting marker-mediated selection of the common oat.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Anna Lyubimova
AU  - Dmitry Eremin
PY  - 2018/12
DA  - 2018/12
TI  - Component composition of oat prolamins in A. strigosa Schreb., A. abyssinica Hochst., A. byzantina C. Koch. and A. sativa L.
BT  - International scientific and practical conference "AgroSMART - Smart solutions for agriculture" (AgroSMART 2018)
PB  - Atlantis Press
SP  - 478
EP  - 482
SN  - 2352-5401
UR  - https://doi.org/10.2991/agrosmart-18.2018.90
DO  - 10.2991/agrosmart-18.2018.90
ID  - Lyubimova2018/12
ER  -