Overexpression of the Tryptophan Cluster in Corynebacterium Glutamicum

DOI

10.2991/ap3er-15.2015.66How to use a DOI?

Keywords

Corynebacterium glutamicum; tryptophan operon; overexpression

Abstract

Extract the total DNA of HYHI, Trp cluster were cloned by PCR according to the DNA of HYHI, the length is 7100bp. Then the gene was connect to the expression vector pZ8-1, recombinant plasmid pZ8-1-Trp cluster was obtained. The plasmid was transferred to HYHI. The positive bacteria were induced by IPTG and determination by SDS-PAGE. The consequence show that the gene cluster is expression. And the same time, anthranilic acid synthase and tryptophan synthetase activity compared with the control increased 2.4 and 3.1 times, respectively

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Hansong Yu
AU  - Xiaodi Liu
AU  - Jiahuan Liu,
AU  - Yuhua Wang
AU  - Yaohui Hu
PY  - 2015/06
DA  - 2015/06
TI  - Overexpression of the Tryptophan Cluster in Corynebacterium Glutamicum
BT  - Proceedings of the 2015 Asia-Pacific Energy Equipment Engineering Research Conference
PB  - Atlantis Press
SP  - 281
EP  - 284
SN  - 2352-5401
UR  - https://doi.org/10.2991/ap3er-15.2015.66
DO  - 10.2991/ap3er-15.2015.66
ID  - Yu2015/06
ER  -