Proceedings of the 2015 Asia-Pacific Energy Equipment Engineering Research Conference

Ganoderic Acid Accumulation and Biosynthetic Gene Expression during Fruiting Body Development in Ganoderma lucidum

Authors
Zhao-Rui Feng, Huan-Jun Li, Jun-Wei Xu
Corresponding Author
Zhao-Rui Feng
Available Online June 2015.
DOI
10.2991/ap3er-15.2015.83How to use a DOI?
Keywords
Ganoderma lucidum; individual ganoderic acid; total triterpenoids; gene expression; fruiting body development
Abstract

Objective: This work aimed to investigate the accumulation of total triterpenoids, individual ganoderic acid (GA) content and expression of four important biosynthetic genes during fruiting body development in Ganoderma lucidum. Methods: Individual GA content was determined by high performance liquid chromatography, and the transcription levels of the GA biosynthetic genes were analyzed by real-time quantitative PCR. Results: The maximum content for total triterpenoids, GA-T, GA-S and GA-Me was 1210, 16.5, 14.3, 12.5 g/ 100 mg dry weight, respectively, at the immature stage. The maximum transcript levels for GA biosynthetic genes encoding 3-hydroxy-3-methylglutaryl-CoA reductase, farnesyl pyrophosphate synthase, squalene synthase and lanosterol synthase were up-regulated by 1.8-, 8.7-, 30.5- and 19.2-fold at the immature stage. Conclusion: The result showed that the content of total triterpenoids and individual GA at the immature stage was higher than that at the primordium and the mature stages. The higher GA content at the immature stage was partially related to increased transcription of these genes. This work will be help for better utilization of fruiting body of G. lucidum and further investigations on the GA biosynthesis regulation.

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

Volume Title
Proceedings of the 2015 Asia-Pacific Energy Equipment Engineering Research Conference
Series
Advances in Engineering Research
Publication Date
June 2015
ISBN
978-94-62520-63-9
ISSN
2352-5401
DOI
10.2991/ap3er-15.2015.83How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Zhao-Rui Feng
AU  - Huan-Jun Li
AU  - Jun-Wei Xu
PY  - 2015/06
DA  - 2015/06
TI  - Ganoderic Acid Accumulation and Biosynthetic Gene Expression during Fruiting Body Development in Ganoderma lucidum
BT  - Proceedings of the 2015 Asia-Pacific Energy Equipment Engineering Research Conference
PB  - Atlantis Press
SP  - 354
EP  - 358
SN  - 2352-5401
UR  - https://doi.org/10.2991/ap3er-15.2015.83
DO  - 10.2991/ap3er-15.2015.83
ID  - Feng2015/06
ER  -