Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)

Electrochemical Study on A Heme-Histidine-Sodium Decyl Sulfate Nano Micelles Complex Artificial Peroxidase

Authors
Di LI, Yushuai ZHANG, Fangyong GENG, Baolin XIAO, Jun HONG, Tao HONG
Corresponding Author
Di LI
Available Online December 2016.
DOI
https://doi.org/10.2991/bep-16.2017.20How to use a DOI?
Keywords
heme; nano micelle; artificial peroxidase; electrochemistry
Abstract

A heme-histidine-sodium decyl sulfate nano micelles complex with peroxidase activity (MAP) was constructed self-assembly and immobilized on a hydroxy fullerene modified glassy carbon electrode. The cyclic voltammograms of the modified electrode with a pair of redox peaks were investigated. The formal potential of the MAP modified electrode was determined to be -0.312V, at a scan rate of 0.05V/s in 50 mmol/L sodium phosphate buffer solution (pH 8.0). The electron transfer rate constant (ks) and cathodic transfer coefficient (α) were 1.96s-1 and 0.4, respectively. The cathodic peak current of the MAP modified electrode responses linearly to hydrogen peroxide ranging from 300 µmol/L to 1700 µmol/L. The detection limit was concluded to be 32 µmol/L. The apparent Michaelis-Menten constant (Kmapp) could be determined to be 1.08 mmol/L.

Copyright
© 2017, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)
Series
Advances in Biological Sciences Research
Publication Date
December 2016
ISBN
978-94-6252-287-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/bep-16.2017.20How to use a DOI?
Copyright
© 2017, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Di LI
AU  - Yushuai ZHANG
AU  - Fangyong GENG
AU  - Baolin XIAO
AU  - Jun HONG
AU  - Tao HONG
PY  - 2016/12
DA  - 2016/12
TI  - Electrochemical Study on A Heme-Histidine-Sodium Decyl Sulfate Nano Micelles Complex Artificial Peroxidase
BT  - Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)
PB  - Atlantis Press
SP  - 95
EP  - 99
SN  - 2468-5747
UR  - https://doi.org/10.2991/bep-16.2017.20
DO  - https://doi.org/10.2991/bep-16.2017.20
ID  - LI2016/12
ER  -