A Sensitive and Reproducible Spectrophotometric Assay for Glucose Oxidase Activity and Its Application in Conformational Lock

DOI

10.2991/bep-16.2017.19How to use a DOI?

Keywords

spectrophotometic assay; glucose oxidase; guaiacol; horseradish peroxidase; coupled system; conformational lock

Abstract

Glucose-oxidase-horseradish peroxidase-guaiacol coupled system was applied for glucose oxidase assay with high sensitivity and prefect reproducibility. The optimum pH value was 5.8. The Michaelis-Menten constant (Km) and Vmax were determined to be 30.0±1.0 mM and 2.49±0.08 µM/s, respectively, for the assay system at 25°C. The relative standard deviation was 2.8% for 31 times measurements of GOD activity. The GOD assay could be applied in conformational lock study on GOD. Results of bio-thermodynamics suggested that the number of contact area of GOD is two, which is consistent with the computation results by using Ligplot and Pymol.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Fangyong GENG
AU  - Di LI
AU  - Yunfei GAO
AU  - Baolin XIAO
AU  - Jun HONG
AU  - Ali Akbar MOOSAVI-MOVAHEDI
PY  - 2016/12
DA  - 2016/12
TI  - A Sensitive and Reproducible Spectrophotometric Assay for Glucose Oxidase Activity and Its Application in Conformational Lock
BT  - Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)
PB  - Atlantis Press
SP  - 88
EP  - 94
SN  - 2468-5747
UR  - https://doi.org/10.2991/bep-16.2017.19
DO  - 10.2991/bep-16.2017.19
ID  - GENG2016/12
ER  -