Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)

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91 articles

A Prebiotic Surface Catalysed Photochemically Activated Synthesis of L-Cysteine

Nigel AYLWARD
Ethyne forms a weak charge-transfer complex with surface catalysts such as Mg.porphin in which the ethyne group has a net positive charge (0.08), and the conjugated porphin has a negative charge. This neutral complex is polarised and undergoes a nucleophilic addition reaction with ammonia at the carbene...

A Novel Biphasic Scaffold with Gradient Pore Structures for Osteochondral Tissue Engineering

Duo PAN, Ruixue YIN, Hongbo ZHANG, Shengbing YANG, Wenjun ZHANG
This study was aimed at using biomimetic strategies to construct a biphasic scaffold. Biphasic scaffolds were constructed using the 3-dimentional printing technology with two phases: the bony phase and the chondral phase. The bony phase was using the composition of poly (lactide-co-glycolide) (PLGA)...

Effects of Light Quality on The Quality Formation of Tomato Fruits

Baoxing XIE, Shiwei SONG, Houcheng LIU, Guangwen SUN, Riyuan CHEN
The effects of light quality on the quality formation of tomato (cv. MicroTom) fruits were studied in controlled-environment house. There were 4 LEDs treatments: the ratio of red (660nm): blue (460nm) was 1:1 (1R1B), 2:1 (2R1B), 4:1 (4R1B) and 8:1 (1R1B), the Photosynthetic Photon Flux Density (PPFD)...

Characterization and Identification of The Key Residues Abound The Activity Site Tunnel of Trehalose Synthase from Pseudomonas Stutzeri Qlu3

Jing SU, Yunxiao ZHANG, Zhenzhen LI, Piwu LI, Lizhen HAO, Haijie ZHENG, Ruiming WANG
The conversion of maltose into trehalose has important applications in the manufacture of food and other products. The enzyme trehalose synthase (TreS) catalyzes the interconversion of maltose and trehalose with glucose as a byproduct. In this study, treS was cloned from Pseudomonas stutzeri Qlu3 genomic...

Effects of Total Flavonoids of Rosa Chinensis on Focal Cerebral Ischemia Reperfusion Model in Mice

Xiaoli YAN, Huisu WU, Xiaoyan FANG, Minsan MIAO
To observe the effects of total flavonoids of Rosa chinensis on focal cerebral ischemia reperfusion model in mice, Method: Nimodipine group, cerebral collaterals group, large, medium and small doses group of the Rosa chinensis total flavonoids were administered with corresponding drugs; Sham operation...

Effects of Propranolol on Osteoclasts Cultured in Vitro

Xiaojuan LI, Xiaorui XIE, Jin REN, Liling REN, Jian CAO
Objective: To study the effect of propranolol on osteoclasts. Methods: (1)propranolol at the doses of 10-2 µmol/ml, 10-3 µmol/ml, 10-4 µmol/ml, were added into the osteoclasts for coculture for 72 h, then tartrate-resistant acid phosphatase (TRAP) and toluidine blue staining...

Synthesis and Performance of A Novel Penetration Enhancer

Qin WU, Lei TANG, Haimin SHEN, Hongxin SHI
Preparation and percutaneous absorption of 2-methyl-2-perfluoro-2'-methyl-3'-oxoca- proyloxyl ethoxycarbonyl ethylmethyldipolyethoxylsilicane (V) were investigated. V was synthesized from 2-perfluoro-2'-methyl-3'-oxocaproyloxylethyl methacrylate and methyldichlorosilicane by hydrosilication, then by...

Numerical and Physical Models of Distal Anastomosis in Coronary Artery Bypass Grafting

Haiyan LONG, Gang YANG
The progressive anastomotic intimal hyperplasia has been considered as a major failure reason for coronary artery bypass grafting (CABG), which is associated with the abnormality of the hemodynamic conditions. We designed a set of plexiglass models which can serve as in vitro cells or tissue culture...

Preliminary Study on The SYBR Green I RT- qPCR for Rabbit Hemorrhagic Disease Virus 2 (RHDV2) Detection

Zexiao YANG, Yadong LIU, Zhengqun MENG, Bo WANG, Xueping YAO, Yin WANG, Yi GENG, Yan LI
To develop a rapid method for the detection RHDV2, 4 specific primers and 12 overlapping PCR primers were designed to amplify the conserved RHDV2 specific DNA fragment according to the genome sequences of RHDV and RHDV2 published in GenBank. Based on the synthesis of a conserved part of the RHDV2 sequence...

Amygdala-Prefrontal Connectivity Analysis of Decoding Human Emotion

Wenjie HE, Haibing BU, Zhonglin LI, Bin YAN, Li TONG, Linyuan WANG
Functional neuroimaging studies have found that emotion network in patients with some psychiatric and neurological diseases was abnormal. However, human emotion network is extremely complex and understanding of it still remains unclear. So, it's difficult to regulate and remodel emotion network directly....

Enhancement of The Edible Mushroom Pleurotus Ostreatus Yield via A New Liquid Fermentation Tank

Xiaoyu DUAN, Yu HU, Yuxian YOU, Qianqian TANG, Yiwen LI, Lan ZHANG, Cheng LI, Yuntao LIU
Nowadays, the edible and medicinal values of edible fungi have been widely recognized all over the world, particularly in China and Japan, the edible fungi have become important ingredients in Traditional Chinese Medicine. Hundreds of species of edible fungi had been studied and confirmed with biological...

Using A New Mushroom Shake Flask Culture Device to Develop Suillellus Luridus

Yu HU, Xiaoyu DUAN, Yuxian YOU, Yingying HE, Yiwen LI, Qianqian TANG, Lan ZHANG, Cheng LI, Daiwen CHEN, Yuntao LIU
A new shake flask culture device was introduced in this paper, whose main part was comprised with mouth, body and bottom. Besides, it also included cork; pump; small bacteria filter and etc. So, the utility model had the advantages of simple structure, artful design, and it was more convenient to use....

Research Status and Development Trend of The Signal Pathway of Allergic Asthma

Hexia LI, Chaopin LI, Xinfeng WANG, Qian LIU
Allergic asthma is a chronic airway inflammatory disease characterized by the infiltration of eosinophils in the lungs and the production of various kinds of stimulating factors, incidence rate showed an upward trend. In this paper, the research status and future development trend of the signal pathway...

A Novel Feature Selection Method for Gene Expression Data Based on Samples Localization

Mingyue SHENG, Wei DU, Yuan TIAN, Yanchun LIANG
It is an important and hot topic for researchers to develop an efficient and robust feature selection method from gene expression profile data with thousands of genes and small sample size. At present, most of feature selection methods are constructed models to use all samples of gene expression data,...

Extracellular Potential Recording of Patterned Rat Olfactory Bulb Neuronal Network Using Planar Microelectrode Arrays

Jian TIAN, Chunlong TU, Bobo HUANG, Xuesong YE
The olfactory bulb is an important structure in olfactory information processing. Exploring electrophysiological characteristics of dissected olfactory bulb neuron in vitro is helpful to improving the understanding of the mechanism of the olfactory information processing. In this work, a novel neural...

ITS and Trnl-F Sequences Analysis of Pulsatilla.Dahurica (Fisch.) Spr.

Tiantian WANG, Guanzhun LIN, Shaoxuan ZHANG, Yarong LI
To provide scientific data of the internal transcribed spacer (ITS) and trnL-F sequences for the authentication of Pulsatilla chinesis (Bge.) Regel., we extracted the genome DNA from the leaves of Pulsatilla.dahurica (Fisch.) Spr., collected in Jilin Province, amplified the ITS and trnL-F region using...

Microencapsulation of DHA Algal Oil by Spray Drying

Wenyao SHAO, Mengwen YAN, Quanling XIE, Xueshan PAN
The aim of this study is to evaluate and optimize the influence of process conditions on the microencapsulation of DHA algal oil by spray drying, while using whey protein isolate (WPI) and maltodextrin (MD) as wall material. Emulsion stability was analyzed. Microencapsulation efficiency, surface oil...

Effect of Compound-Maqin-Decoction on P38MAPK and Foxp3 Protein Expression of Lung Issue in Asthmatic Rats

Peng QIAN, Zhaoxia XU, Xueping LI, Sijing WANG
OBJECTIVE: To observe the effect of Compound-Maqin-Decoction (CMD) on p38MAPK and Foxp3protein expression of lung issue in asthmatic rats. METHODS: The asthmatic rat model was established by ovalbumin and was intervened with CMD. After inducing asthma and four-week treatment, the rats were sacrificed...

A Sensitive and Reproducible Spectrophotometric Assay for Glucose Oxidase Activity and Its Application in Conformational Lock

Fangyong GENG, Di LI, Yunfei GAO, Baolin XIAO, Jun HONG, Ali Akbar MOOSAVI-MOVAHEDI
Glucose-oxidase-horseradish peroxidase-guaiacol coupled system was applied for glucose oxidase assay with high sensitivity and prefect reproducibility. The optimum pH value was 5.8. The Michaelis-Menten constant (Km) and Vmax were determined to be 30.0±1.0 mM and 2.49±0.08 µM/s, respectively,...

Electrochemical Study on A Heme-Histidine-Sodium Decyl Sulfate Nano Micelles Complex Artificial Peroxidase

Di LI, Yushuai ZHANG, Fangyong GENG, Baolin XIAO, Jun HONG, Tao HONG
A heme-histidine-sodium decyl sulfate nano micelles complex with peroxidase activity (MAP) was constructed self-assembly and immobilized on a hydroxy fullerene modified glassy carbon electrode. The cyclic voltammograms of the modified electrode with a pair of redox peaks were investigated. The formal...

Conversion of Fructose into Levulinic Acid by Catalysis of Ionic Liquid

L. LIU, H. REN
76% Yield of levulinic acid was obtained from fructose conversion catalyzed by acidic ionic liquid in water. The chemical structures and morphologies of humin byproduct were investigated, indicating three stages of transition for the first time. At the early stage, 5-hydroxymethylfurfural (HMF) intermediate...

A Method for Blink Artifact Detection and Removal with Wavelet Transform and Hilbert Transform

Xiaobai CAI, Junjun CHEN
This paper presents a method of blink artifact removal of single lead EEG signal. After a wavelet transform signal singularity points are detected and modulus maxima values of wavelet transform are applied to find peaks of blink artifacts. By using Hilbert transform instantaneous frequency is obtained....

Microbial Production of Xylitol from D-arabitol by Gluconobacter Oxydans

Huanhuan ZHANG, Junhua YUN, Tinashe Archbold MAGOCHA, Miaomiao YANG, Yanbo XUE, Xianghui QI
Xylitol, a five carbon sugar alcohol, has numerous applications in the fields of food and pharmaceuticals. Gluconobacter oxydans contains two enzymes which are membrane-bound D-arabitol dehydrogenase and soluble xylitol dehydrogenase enabling the production of xylitol from D-arabitol. This review provides...

The Synthesis of Diazinon with The Catalysis of Ionic Liquid under The Condition of Microwave

Lin HU, Yegui WANG, Xiaoqin ZHOU, Juan TANG, Jingqing LI, Kuijiang WONG, Wenyuan XU
A novel synthetic methods for the efficient and environmentally-friendly production will provide useful inspiration. This report presents a novel method for microwave-assisted synthesis of diazinon with different organic solvents, microwave heating power, heating time, catalysts. The optimal conditions...

N-Glycosylation Sited at Residues of Catalytic Center Could Repress The Activity of Endoglucanase from Rhizopus Stolonifer

Bin TANG, Yingying ZHANG, Wei CHENG, Qingqing ZHANG, Song LI
As a common post-translational modification of proteins, glycosylation has been proven effectively influence the activity of enzyme. To improve the activity of endoglucanase (EGII) from Rhizopus stolonifer TP-02, we studied the effect of glycosylation on the structure and activity of EGII. Two potential...

One New Monoterpenoid from the Fruit of Gardenia Jasminoides

Yueming ZUO, Zhongli ZHANG
One new monoterpenoid, 5-aldehyde-6'-O-sinapoyljasminoside A (1), together with two known compounds 6'-O-sinapoyljasminoside B(2) and 6'-O-trans-sinapoyljasminoside L(3), were isolated from the fruits of Gardenia jasminoides Ellis. The structures of the new monoterpenoid compound were elucidated by means...

Preliminary Study on The RT-LAMP Assay for Rabbit Hemorrhagic Disease Virus Type 2 Detection

Zexiao YANG, Yilin LUO, Zhengqun MENG, Xueping YAO, Han ZHANG, Yin WANG, Xiaoxue XIANG, Lijun ZHOU, Yadong LIU, Yan LI
To develop a rapid molecular biological method for the detection of rabbit hemorrhagic disease virus type 2 (RHDV2), a set of 4 primers were designed according to the conserved sequence fragment of the capsid protein(VP60) gene of RHDV2 published in GenBank, and the reverse transcriptase loop-mediated...

Study on The Mildewproof Method of Fresh Xinhui Citrus Peels

Chen LI, Yingzhu WU, Jingyi MA, Leyu MA, Ning QING
To explore the effect to storage method of fresh Xinhui citrus peels by using against storage and ethanol mildewproof method, the main fungus which caused the mildew of fresh peels was also checked. The effect of hance ginger on peel moldy was observed by changing the humidity of storage environment...

The Microbial Community in A Chinese Traditional Fermented Suanrou by High Throughput Sequencing

Ping HU, Yun CHEN, Jianlong ZHAN, Yulong ZHANG
Microbial diversity in a Chinese traditional spontaneous fermented sour meat product, Suanrou, was investigated using High-throughput sequencing. A taxonomic analysis was carried out on 1248 OTUs obtained with 454-pyrosequencing of 16S rRNA amplicons of the bacterial microbiota in 3samples of suanrou...

Three Monoterpenoids Eucommiol from The Leaves of Eucommia Ulmoides Protect against Neurotoxicity in PC12 Cells

Yueming ZUO, Zhongli ZHANG
One new monoterpenoid compound 4'-acetyl-eucommiol (1) and two known compounds eucommiol(2), 1-deoxyeucommiol(3) were isolated from the leaves of Eucommia ulmoides. The structures of the new monoterpenoid eucommiol were elucidated by means of chemical evidence and 1D-and 2D-NMR (1H, 13C, HSQC, HMBC,...

Integrated Analysis of miRNA and Gene Networks on Cancer Expression Data

Yuping LI, Wei DU, Lihua HE, Zhongbo CAO, Yanchun LIANG
MircroRNAs (miRNAs) are one type of small non-coding RNAs and play important roles in regulating wide range of biological processes, such as stem cell maintenance, tissue development and cell metabolism. By modulating these key cellular processes, miRNAs can simultaneously regulate both oncogenes and...

The Phase of Oscillation in Cortical Network and Its Relationship with Firing Rate and Neuronal Activation

Daming WANG, Yaoru SUN
Gamma-band neuronal oscillation and synchronization (30-90 Hz) are frequently observed in several cortical and subcortical circuits and are supposed to be involved in a variety of cognitive functions. The phase of the oscillation, referred to as one aspect of communication through coherence (CTC) hypothesis,...

Spectrophotometric Determination of Penicillamine in Pharmaceutical Sample using Fe(III)-Tiron System

Xinrong WEN, Changqing TU
Hydrosulfuryl(-SH) in penicillamine molecule can reduce Fe(III) to Fe(II), then using tiron as chromogenic reagent of Fe(III), and the content of penicillamine is determinated indirectly through determinating the surplus content of Fe(III) in the system. An accurate fast spectrophotometric method for...

Chitosan Derivative Nanoparticles as Prolonged Releasing Drug Carriers

Bohua FENG, Liufen PENG
Ricinoleic acid grafted amphiphilic carboxymethy chitosan (CMC-g-RA) was synthesized as a carrier to load botanical drug rotenone (Rot). Then Rot/CMC-g-RA water dispersion in nanoscale was prepared, whose shape, zeta potential, loading efficiency and outdoor stability were characterized accordingly....

Research and Implementation of Discovering and Recommending Experts in Medical Community-Based Q&A

Lan FANG, Yu FANG
With the continuous development of the Internet, community-based Q&A (CQA) system has become an effective platform for people to access online information and help. People can submit their own question in CQA and get answers from others. There will be an enormous amount of questions all the time, and...

Prognostic Analysis of The Effects of Starvation and Overfeeding on Children

Le KANG, Mingsan MIAO
By comparing the effects of excess nutrients and malnutrition on the body of children, It is concluded that the harm to children's body caused by excessive nutrition is more than that of malnutrition, and overnutrition is also a performance of malnutrition. The causes of obesity in children, the present...

Experimental Study on Carbonation Treatment

Zhenwei YU, Kunjie CHEN, Wang YU, Zhao LI
The scope of Chinese medicine residue and utilization is relatively narrow, and the harmless processing technology needs to be systematized. Since its feasibility is still being questioned by people, this research discusses the properties of the Chinese medicine residue carbonation characteristics and...

Progress in Research on Small Molecules as Gene-targeted Drugs

Yanli WANG, Sijia WANG, Jun XU, Wen ZHANG
The findings of effective targets and the identification of target binding molecules are two key points in drug discovery. The complete human genome and their rapid increase in structural and functional information led to new possibilities for diagnosis and therapy of diseases, using gene-targeted drugs....

A Recurrence Quantification Analysis of Traditional Chinese Medicine Pulse and Its Application in Assessment of Coronary Artery Disease

Rui GUO, Yiqin WANG, Jianjun YAN, Haixia YAN
Purpose: This study was conducted to illustrate that pulse diagnosis of traditional Chinese medicine (TCM) was useful in the assessment of coronary artery lesions in patients with Coronary Heart Disease (CHD). Methods: By using phase-space reconstruction, one-dimensional pulse signals of participants...

Melittin: A Key Composition of Honey Bee Venom with Diverse Pharmaceutical Function

Sheng HUANG, Jianhua WANG, Xiaozhong WANG, Chenghong LI
Melittin is the main toxic component in the the honey bee venom. It is a cationic, lytic peptide constituted by 26 amino acid residues. This peptide is an amphoteric molecule which the carboxy-terminal region is hydrophilic and the amino-terminal region is hydrophobic due to the presence of a series...

Quantitative Analysis of Ketoprofen Enantiomers by Liquid Chromatography/Electrospray Ionization-Mass Spectrometry

Fen YANG, Dongri JIN
A enantioselective and sensitive liquid chromatography/electrospray ionization-mass spectrometry (LC/ESI-MS) method is developed for the quantitative analysis of ketoprofen (KET) enantiomers based on a straightforward one-pot two-step stable isotope-coded derivatization of KET with a chiral derivatization...

Tongue Characteristics of Different TCM Syndromes on Asthma

Sijing WANG, Zhaoxia XU, Yiqin WANG
Asthma is kind of disease affecting quality of life. Different TCM asthma types have different characteristics.TCM tongue images can be objective indexes for asthma TCM differentiation and therapeutic evaluation. More and more modern technique and analysis methods are used to discuss meaning of tongue....

Blends of Poly (L-Lactic Acid) and Glucose Prepared from Mixture Solution

Dan CAO, Qinwei GAO, Yinping ZHAO, Wei MING
Poly (L-lactic acid) (PLLA) and glucose (Glu) were respectively dissolved in N, N-dimethyl acetamide to prepare solutions. The blends of PLLA and glucose with various ratios were prepared by mixed solution method, and characterized with infrared Fourier transform spectrometry, differential scanning calorimetry,...

Crystallization of the Racemic Conglomerate and Racemic Compound of Efonidipine

Linyuan ZHU, Zhanjun LI, Yuzhen YU, Wen LI
Efonidipine is a dual blocker of T-type and L-type calcium channels with 1, 4-dihydropyridine structure attached to a phosphonate skeleton. As a chiral compound, it has a special property that can exist as a racemic conglomerate or a racemic compound based on the different crystallization conditions....

Synthesis of Dinucleoside Triphosphates Containing Antiviral Nucleoside

Rong LIU, Shanshan GONG
A general and efficient method for the synthesis of asymmetrical dinucleoside triphosphates with an antiviral nucleoside moiety has been developed. The coupling of adenosine diphosphate with antiviral nucleoside phosphoropiperidate via the DCI activation of P-N bond afforded the desired products in good...

Cooking Fumes and Relative Diseases

Chunyan WANG, Lifang LIU, Guoping HE
Cooking fumes are mixture of various toxic components such as aldehydes, heterocyclic amines (HCAs), polycyclic aromatic hydrocarbons (PAHs), fat aerosols and particulate matters (PM), which are mainly produced during or after cooking. Due to the toxic contents of CFs, CFs might cause lung toxicity,...

Evaluation of Heavy Metal Pollution Prevention Technology based on AHP-FCE

Linan SHAO, Guoqiang CHEN
AHP-FCE model was introduced to carry out evaluation of heavy metal pollution prevention technology. AHP was used to determine the weights of various evaluation indices, and FCE was used to evaluate heavy metal pollution prevention technology. The paper took HDS technology. as an example, to introduce...

A Comparison of the Oxygen Release from Roots of Thalia and Pontederia in Constructed Wetland Wastewater Treatment Systems

Chan DONG, Yinghao HUANG
To investigate the ability of oxygen release from roots of different vegetation, and explain the mechanism. This paper tested the variation of oxygen release rate from roots of Thalia and Pontederia using a titanium ( ) citrate buffer. The results shows that oxygen release rates with the same condition...

Soil Respiration and Photosynthesis of Leaves in Greenhouse Cultivation Cherry Tomato

Yinli LIANG, Tianli BAO, Lan MU
To explore the optimum growth condition of cherry tomato (Lycopersiconesculentum), the cherry tomato was used to study the soil respiration and photosynthesis of leaves under greenhouse and outdoor condition with Lou soil and loessial soil during fruiting period in the year 2013 and 2014.The results...

Exploration of Chinese Ecological and Environmental Philosophy

Shaoyao HE, Duo LI, Ouyang DUO
Chinese traditional culture contains rich ecological philosophy thought. This paper focuses on the analysis of the ecological and environmental philosophy of "harmony between man and nature" in Chinese ancient Confucianism and Taoism. It is believed that the thought has important practical guiding function...