The effective part of Dioscorea nipponica Makino on esophageal carcinoma cells and the interaction with human serum albumin

DOI

10.2991/bep-16.2017.91How to use a DOI?

Keywords

Dioscorea nipponica Makino, Esophageal carcinoma cell, MTT, Human serum albumin

Abstract

The effective part (BESDM) was extracted from the DioscoreaÿnipponicaÿMakino. Cytotoxicity of the BESDM on Ec9706, Eca109 and TE1 cells tested by MTT assay. The result showed the Ic50 values of Ec9706, Eca109 and TE1 are 7.87ñ0.08, 3.76ñ0.04 and 13.53ñ0.06 gúml-1, respectively. Fluorescence was used to study the interaction of BESDM with human serum albumin (HSA). The intrinsic fluorescence of HSA was quenched by BESDM. The quenching mechanism is mainly static quenching. The thermodynamic parameters, H (-1656.79 kJúmol-1), S (-5381.62 Júmol-1ú K-1) and G (-26.16 to -27.69 kJúmol-1), indicated that the Hydrogen bonding and van der Waals force played major roles. The binding constant (Kb) were 4.4545ž108 Lúmol-1 (303 K) and 3.4834ž103 Lúmol-1 (313 K).

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Fuchun SI
AU  - Wenbin WANG
PY  - 2016/12
DA  - 2016/12
TI  - The effective part of Dioscorea nipponica Makino on esophageal carcinoma cells and the interaction with human serum albumin
BT  - Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)
PB  - Atlantis Press
SP  - 407
EP  - 411
SN  - 2468-5747
UR  - https://doi.org/10.2991/bep-16.2017.91
DO  - 10.2991/bep-16.2017.91
ID  - SI2016/12
ER  -