EACHL Effect on esophageal carcinoma cells and the interaction with bovine serum albumin

DOI

10.2991/bep-16.2017.90How to use a DOI?

Keywords

Chinese honey locus; Esophageal carcinoma cell; MTT, Bovine serum albumin

Abstract

EACHL was extracted from the Chinese honey locust. MTT assay was used to test the cytotoxicity of the extracts on esophageal carcinoma cells. The result of MTT assay showed the Ic50 values of Eca109 and TE1 are 11.53, 32.07 g-ml-1, respectively. The interaction of EACHL with bovine serum albumin (BSA) was studied at pH 7.40 (ionic strength 0.10 mol-L-1) by fluorescence. EACHL can quench the intrinsic fluorescence of BSA, the quenching mechanism is static quenching. The thermodynamic parameters, H (-1175.43 kJ-mol-1), S (-4035.13 J-mol-1- K-1) and G (-47.22 to -11.99 kJ-mol-1), indicated that the Hydrogen bonding and van der Waals force played major role. The binding constant (Kb) were 1.3816ž108 L-mol-1 (303 K) and 1.0010ž102 L-mol-1 (313 K). Synchronous fluorescence studies showed that the conformation of BSA could be changed during EACHL was added.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Fuchun SI
AU  - Wenbin WANG
PY  - 2016/12
DA  - 2016/12
TI  - EACHL Effect on esophageal carcinoma cells and the interaction with bovine serum albumin
BT  - Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)
PB  - Atlantis Press
SP  - 403
EP  - 406
SN  - 2468-5747
UR  - https://doi.org/10.2991/bep-16.2017.90
DO  - 10.2991/bep-16.2017.90
ID  - SI2016/12
ER  -