Pomegranate Extract Protects Endothelial Cells from TNF-α Associated Damage

Ravindran Vini; Sreeja Sreekumar; Juberiya M. Azeez; Sreeja Sreeharshan

doi:10.2991/978-94-6463-020-6_27

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Pomegranate Extract Protects Endothelial Cells from TNF-α Associated Damage

Authors

Ravindran Vini¹^{, 2}, Sreeja Sreekumar³, Juberiya M. Azeez¹^{, 2}, Sreeja Sreeharshan¹^{, *}

¹Cancer Research Program, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India

²Research Centre, University of Kerala, Thiruvananthapuram, India

³Cardiff Oncology, San Diego, CA, USA

^*Corresponding author. Email: ssreeja@rgcb.res.in

Corresponding Author

Sreeja Sreeharshan

Available Online 14 December 2022.

DOI: 10.2991/978-94-6463-020-6_27 How to use a DOI?
Keywords: Pomegranate; apoptosis; atherosclerosis; Reactive Oxygen Species
Abstract: Pomegranates are known for being rich in polyphenols and are considered to have immense therapeutic potential. The present study investigates the hypothesis that the Methanolic Extract of Pomegranate (PME), a rich source of antioxidants, may reverse the adverse effects of TNF-α in endothelial cells. This was done by pre-treating the endothelial cells EA.hy926 with PME (80 µg/ml) before subjecting them to apoptotic stimuli, which was TNF-α in combination with cyclohexamide. PME was found to rescue a population of cells from apoptosis induced by TNF-α modulating the levels of BCL2 and BAX involved in intrinsic apoptotic pathway. PME was found to increase the BCL-2/BAX ratio and reverse the elevated levels of effector caspase and thus assist cells to escape from apoptotic stimuli. Also, the extract was found to attenuate oxidative stress by reducing the levels of Reactive Oxygen Species (ROS). Supplementing its anti-atherosclerotic potential, PME pre-treatment diminished the elevated levels of adhesion molecules like VCAM upon TNF-α treatment. PME may therefore have therapeutic implications in protecting the endothelium from TNF-α triggered atherosclerosis.
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title: Proceedings of the conference BioSangam 2022: Emerging trends in Biotechnology (BIOSANGAM 2022)
Series: Advances in Biological Sciences Research
Publication Date: 14 December 2022
ISBN: 978-94-6463-020-6
ISSN: 2468-5747
DOI: 10.2991/978-94-6463-020-6_27 How to use a DOI?
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

ris enw bib

TY  - CONF
AU  - Ravindran Vini
AU  - Sreeja Sreekumar
AU  - Juberiya M. Azeez
AU  - Sreeja Sreeharshan
PY  - 2022
DA  - 2022/12/14
TI  - Pomegranate Extract Protects Endothelial Cells from TNF-α Associated Damage
BT  - Proceedings of the conference BioSangam 2022: Emerging trends in Biotechnology (BIOSANGAM 2022)
PB  - Atlantis Press
SP  - 276
EP  - 289
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-020-6_27
DO  - 10.2991/978-94-6463-020-6_27
ID  - Vini2022
ER  -

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