Clitoria ternatea Linn Extract as Natural pH Indicator in Mannitol Salt Agar Medium
- https://doi.org/10.2991/ahsr.k.200523.078How to use a DOI?
- Clitoria ternatea Linn, pH indicator, bacteria differentiation medium
Objectives: Mannitol Salt Agar (MSA) medium used to distinguish mannitol fermented ability among Staphylococcus species. The ability to ferment mannitol visualized by color changing in agar medium. Fenol red is color indicator usually used in standard MSA medium which has ability to turn from red to yellow in acid pH. This study aim is to modify the MSA medium using Clitoria ternatea Linn flower extract as natural pH indicator. The flower dissolved in boiled distilled water and filtrated. Modified MSA made by 10% of C. ternatea Linn flower extract content. Staphylococcus aureus and Staphylococcus epidermidis were used as tested bacteria. The result showed the color changing in modified MSA agar medium from blue to purple in medium which inoculated by S. aureus, but not showed color changing in medium which inoculated by S. epidermidis. This result is appropriate with the standard medium, which the S. aureus has ability to ferment mannitol causing the color changing in MSA medium. The conclusion of this study is the modified MSA agar medium that contain C. ternatea Linn flower extract has ability to distinguish mannitol fermented bacteria and can used as MSA alternative formula.
- © 2020, the Authors. Published by Atlantis Press.
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Cite this article
TY - CONF AU - Dewi Peti Virgianti AU - Rochmanah Suhartati AU - Khusnul PY - 2020 DA - 2020/06/08 TI - Clitoria ternatea Linn Extract as Natural pH Indicator in Mannitol Salt Agar Medium BT - The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019) PB - Atlantis Press SP - 326 EP - 328 SN - 2468-5739 UR - https://doi.org/10.2991/ahsr.k.200523.078 DO - https://doi.org/10.2991/ahsr.k.200523.078 ID - Virgianti2020 ER -