The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)

Clitoria ternatea Linn Extract as Natural pH Indicator in Mannitol Salt Agar Medium

Authors
Dewi Peti Virgianti, Rochmanah Suhartati, Khusnul
Corresponding Author
Dewi Peti Virgianti
Available Online 8 June 2020.
DOI
https://doi.org/10.2991/ahsr.k.200523.078How to use a DOI?
Keywords
Clitoria ternatea Linn, pH indicator, bacteria differentiation medium
Abstract

Objectives: Mannitol Salt Agar (MSA) medium used to distinguish mannitol fermented ability among Staphylococcus species. The ability to ferment mannitol visualized by color changing in agar medium. Fenol red is color indicator usually used in standard MSA medium which has ability to turn from red to yellow in acid pH. This study aim is to modify the MSA medium using Clitoria ternatea Linn flower extract as natural pH indicator. The flower dissolved in boiled distilled water and filtrated. Modified MSA made by 10% of C. ternatea Linn flower extract content. Staphylococcus aureus and Staphylococcus epidermidis were used as tested bacteria. The result showed the color changing in modified MSA agar medium from blue to purple in medium which inoculated by S. aureus, but not showed color changing in medium which inoculated by S. epidermidis. This result is appropriate with the standard medium, which the S. aureus has ability to ferment mannitol causing the color changing in MSA medium. The conclusion of this study is the modified MSA agar medium that contain C. ternatea Linn flower extract has ability to distinguish mannitol fermented bacteria and can used as MSA alternative formula.

Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

Volume Title
The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)
Series
Advances in Health Sciences Research
Publication Date
8 June 2020
ISBN
978-94-6252-976-2
ISSN
2468-5739
DOI
https://doi.org/10.2991/ahsr.k.200523.078How to use a DOI?
Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Dewi Peti Virgianti
AU  - Rochmanah Suhartati
AU  - Khusnul
PY  - 2020
DA  - 2020/06/08
TI  - Clitoria ternatea Linn Extract as Natural pH Indicator in Mannitol Salt Agar Medium
BT  - The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)
PB  - Atlantis Press
SP  - 326
EP  - 328
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.200523.078
DO  - https://doi.org/10.2991/ahsr.k.200523.078
ID  - Virgianti2020
ER  -