Clitoria ternatea Linn Extract as Natural pH Indicator in Mannitol Salt Agar Medium

DOI

10.2991/ahsr.k.200523.078How to use a DOI?

Keywords

Clitoria ternatea Linn, pH indicator, bacteria differentiation medium

Abstract

Objectives: Mannitol Salt Agar (MSA) medium used to distinguish mannitol fermented ability among Staphylococcus species. The ability to ferment mannitol visualized by color changing in agar medium. Fenol red is color indicator usually used in standard MSA medium which has ability to turn from red to yellow in acid pH. This study aim is to modify the MSA medium using Clitoria ternatea Linn flower extract as natural pH indicator. The flower dissolved in boiled distilled water and filtrated. Modified MSA made by 10% of C. ternatea Linn flower extract content. Staphylococcus aureus and Staphylococcus epidermidis were used as tested bacteria. The result showed the color changing in modified MSA agar medium from blue to purple in medium which inoculated by S. aureus, but not showed color changing in medium which inoculated by S. epidermidis. This result is appropriate with the standard medium, which the S. aureus has ability to ferment mannitol causing the color changing in MSA medium. The conclusion of this study is the modified MSA agar medium that contain C. ternatea Linn flower extract has ability to distinguish mannitol fermented bacteria and can used as MSA alternative formula.

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Dewi Peti Virgianti
AU  - Rochmanah Suhartati
AU  - Khusnul
PY  - 2020
DA  - 2020/06/08
TI  - Clitoria ternatea Linn Extract as Natural pH Indicator in Mannitol Salt Agar Medium
BT  - The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)
PB  - Atlantis Press
SP  - 326
EP  - 328
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.200523.078
DO  - 10.2991/ahsr.k.200523.078
ID  - Virgianti2020
ER  -