Proceedings of the 2015 AASRI International Conference on Circuits and Systems

Chemical Mutagenesis Breeding of Protoplast of Ammonia-oxidizing Bacteria

Authors
Dong Yuwei, Miao Jingzhi, Tang Shirong, Chen Shanglong, Wu Yonghua
Corresponding Author
Dong Yuwei
Available Online August 2015.
DOI
https://doi.org/10.2991/cas-15.2015.22How to use a DOI?
Keywords
ammonia-oxidizing bacteria; protoplast; mutagenesis; lithium chloride; ethidium bromide.
Abstract
Strain with strong capacity of ammoxidation screened by repeated enrichment and separation methods from activated sludge which was collected from sewage treatment plant in China university of mining and technology. Lithium chloride and ethidium bromide mutagenesis methods of ammonia-oxidizing bacteria protoplast were used to breed mutant strain with strong capacity of ammoxidation. The relationship between concentrations of chemical mutagens and capacity of ammoxidation were researched. The results showed that regenerated colony number of protoplast decreased with increased lithium chloride or ethidium bromide mutagenic concentration. After culvation of 1L simulation sewage medium, the mutagenic strain LC002, mutated by 2.5 mg/mL lithium chloride, still had better capacity of ammoxidation with 86.89% removal rate of ammonia nitrogen. The mutagenic strain EB003, mutated by 2 g/mL ethidium bromide, also had better capacity of ammoxidation with 85.67% removal rate of ammonia nitrogen. Protoplast mutagenisis was an effective breeding method to breed excellent ammonia-oxidizing bacteria for further application.
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Proceedings
2015 AASRI International Conference on Circuits and Systems (CAS 2015)
Part of series
Advances in Computer Science Research
Publication Date
August 2015
ISBN
978-94-62520-74-5
ISSN
2352-538X
DOI
https://doi.org/10.2991/cas-15.2015.22How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Dong Yuwei
AU  - Miao Jingzhi
AU  - Tang Shirong
AU  - Chen Shanglong
AU  - Wu Yonghua
PY  - 2015/08
DA  - 2015/08
TI  - Chemical Mutagenesis Breeding of Protoplast of Ammonia-oxidizing Bacteria
BT  - 2015 AASRI International Conference on Circuits and Systems (CAS 2015)
PB  - Atlantis Press
SN  - 2352-538X
UR  - https://doi.org/10.2991/cas-15.2015.22
DO  - https://doi.org/10.2991/cas-15.2015.22
ID  - Yuwei2015/08
ER  -