Dynamic Model of Paclitaxel Biosynthesis Suggests That the Key Enzyme Is Taxadiene 5alpha-Hydroxylase in Taxuschinensis Cell Suspension Culture

DOI

10.2991/cisia-15.2015.195How to use a DOI?

Keywords

paclitaxel biosynthesis genes; relative expression; rate-limiting step

Abstract

Taxus suspension cell cultures are a sustainable source of paclitaxel. In this study, transcript profiles of T.chinensis cells were analysed systematically by RT-qPCR to investigate the transcriptional dynamics of Taxus cell during cultures. Correlation between the expression levels of genes involved in paclitaxel biosynthesis enzymes and the specific synthesis rate of paclitaxel in suspension culture of Taxuschinensishad been studied by a statistic model. The model suggested that, taxadiene 5-alpha-hydroxylase, with the highest correlation coefficient,was the rate-limiting enzyme in paclitaxel biosynthetic pathway in Taxuschinensis.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - S.S Zhu
AU  - Z.Q Miao
PY  - 2015/06
DA  - 2015/06
TI  - Dynamic Model of Paclitaxel Biosynthesis Suggests That the Key Enzyme Is Taxadiene 5alpha-Hydroxylase in Taxuschinensis Cell Suspension Culture
BT  - Proceedings of the International Conference on Computer Information Systems and Industrial Applications
PB  - Atlantis Press
SP  - 717
EP  - 719
SN  - 2352-538X
UR  - https://doi.org/10.2991/cisia-15.2015.195
DO  - 10.2991/cisia-15.2015.195
ID  - Zhu2015/06
ER  -