Proceedings of the 2nd International Conference on Civil, Materials and Environmental Sciences

Optimization of Fermentation Parameters for Laccase Production by a Novel Deuteromycete Fungus Myrothecium Verrucaria NF-05 Using Response Surface Methodology

Authors
Dan Zhao, Yanyang Sun, Renpeng Du, Li Zhang, Jingping Ge
Corresponding Author
Dan Zhao
Available Online April 2015.
DOI
10.2991/cmes-15.2015.118How to use a DOI?
Keywords
Laccase; Deuteromycete; Medium; Optimization; Response-surface methodology
Abstract

To obtain the maximum production of laccase from a novel deuteromycete fungus Myrothecium verrucaria NF-05, optimization of fermentation parameters was performed. Central composite design and response surface analysis revealed the optimum concentration of glucose, CuSO4 and gallic acid to be 26.47 g L-1, 236.3 µM, and 138.4 µM, respectively. This optimization strategy led to the enhancement of laccase production from 12.00 to 19.94 U mL-1, 1.66-fold increase.

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2nd International Conference on Civil, Materials and Environmental Sciences
Series
Advances in Engineering Research
Publication Date
April 2015
ISBN
10.2991/cmes-15.2015.118
ISSN
2352-5401
DOI
10.2991/cmes-15.2015.118How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Dan Zhao
AU  - Yanyang Sun
AU  - Renpeng Du
AU  - Li Zhang
AU  - Jingping Ge
PY  - 2015/04
DA  - 2015/04
TI  - Optimization of Fermentation Parameters for Laccase Production by a Novel Deuteromycete Fungus Myrothecium Verrucaria NF-05 Using Response Surface Methodology
BT  - Proceedings of the 2nd International Conference on Civil, Materials and Environmental Sciences
PB  - Atlantis Press
SP  - 425
EP  - 427
SN  - 2352-5401
UR  - https://doi.org/10.2991/cmes-15.2015.118
DO  - 10.2991/cmes-15.2015.118
ID  - Zhao2015/04
ER  -