A Comparative Study on the Interference Microscopy Imaging and Diffraction Microscopy Imaging for Biological Cells

DOI

10.2991/cmes-15.2015.153How to use a DOI?

Keywords

Phase microscopy imaging, Biological cells, Interference, Diffraction, Comparative study

Abstract

The studying standard of life sciences and clinical medicine is determined by the development level of morphology detection of biological cells. Currently, there are two approaches for revealing the 3D structure of biological cells, namly, interferometric technique and diffractive technique. Both methods could offer the phase information of the cell. Due to the translucent characteristics of cells and the wave nature of light based on cell scale, interference effect coexists with the diffraction effect when light passes through the cells. In this paper, models based on the shape of the typical blood cells are built and simulation experiments of interference as well as diffraction phase imaging are carried out with the models. Furthermore, the similarities and differences between them are discussed, with respect to the intensity distribution and phase distribution. The result agrees well with the experiments. It is suggested to be useful to promote the research of phase recovery methods of the two kinds of imaging technology.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Minjie Liang
AU  - Ying Ji
AU  - Yuanyuan Xu
AU  - Tingting Hua
AU  - Yawei Wang
PY  - 2015/04
DA  - 2015/04
TI  - A Comparative Study on the Interference Microscopy Imaging and Diffraction Microscopy Imaging for Biological Cells
BT  - Proceedings of the 2nd International Conference on Civil, Materials and Environmental Sciences
PB  - Atlantis Press
SP  - 551
EP  - 554
SN  - 2352-5401
UR  - https://doi.org/10.2991/cmes-15.2015.153
DO  - 10.2991/cmes-15.2015.153
ID  - Liang2015/04
ER  -