Cell Fusion and Distinction between Viable Cell and Non-Viable Cell Using Dielectrophoresis and Optical Tweezers

DOI

10.2991/eame-15.2015.16How to use a DOI?

Keywords

optical tweezers; dielectrophoresis; yeast;protoplast; fusion lab-on-chip system

Abstract

A lot of researchers have studied cell fusion and manipulation during the past decades, and this field was pursued low cost and small setup. There is a lot of ways that can manipulate cell such as optical tweezers and dielectrophoresis (DEP). Particularly, optical tweezers can manipulate cells with non-contact and non-invasive so this technique was noticed in biotechnology. In this paper we introduce three techniques based on DEP and optical tweezers: Distinction between viable cells and non-viable cells using DEP, Manipulating cells captured by positive DEP (pDEP) using optical tweezers and PEG fusion combining optical tweezers and DEP. About cell fusion, we would suggest efficient PEG fusion that can fuse cell in selective. We are considering a variety of system combined DEP and optical tweezers now. The systems will be useful to perform cell operation and other applications.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Y. Mizuta
AU  - H. Takuya
AU  - T.D. Nguyen
AU  - K. Taguchi
PY  - 2015/07
DA  - 2015/07
TI  - Cell Fusion and Distinction between Viable Cell and Non-Viable Cell Using Dielectrophoresis and Optical Tweezers
BT  - Proceedings of the 2015 International Conference on Electrical, Automation and Mechanical Engineering
PB  - Atlantis Press
SP  - 59
EP  - 62
SN  - 2352-5401
UR  - https://doi.org/10.2991/eame-15.2015.16
DO  - 10.2991/eame-15.2015.16
ID  - Mizuta2015/07
ER  -