Expression of Beta-Adrenergic Receptor in Glioma LN229 Cells and Its Effect on Cell Proliferation
Junhong Dong, Ping Wang, Qingluan Xu, Kunpeng Lv, Yu Wang
Available Online February 2017.
- https://doi.org/10.2991/emcm-16.2017.140How to use a DOI?
- Glioma; Adrenergic receptor; Proliferation; Norepinephrine; Stress hormone
- Objective To investigate the expression of beta-adrenergic receptor( -AR) in glioma cells and the effect of blocking -adrenergic receptors on glioma cell proliferation. Methods: The expression of beta-adrenergic receptor in glioma LN229 and U251 cells were detected by real time PCR and Western Blotting. The proliferation of glioma cells was analyzed by MTT assay after they were exposed to norepinephrine and propranolol(non-selective blocker of beta adrenergic receptors) alone, or in combination, respectively. Results: The LN229 and U251 cells both expressed beta 1 and beta 2 adrenergic receptors, but beta2-receptor showed a relatively strong expression while beta1-receptor showed a relatively weak expression in LN229cells. The proliferation of glioma cells was significantly inhibited by propranolol which acts as a nonspecific blocker of beta-adrenergic receptors, with a certain time and concentration-dependent matter toward higher inhibition, and the differences had statistically significance (all P <0.05). The proliferation LN299 cells were significantly increased after norepinephrine, which was in a concentration-dependent manner antagonized by propranolol co treatment (P <0.05). Conclusion: Both adrenergic beta1 and beta2 are expression of glioma cells, and non-selective blockage of beta adrenergic receptor can effectively inhibit the proliferation of glioma cells.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Junhong Dong AU - Ping Wang AU - Qingluan Xu AU - Kunpeng Lv AU - Yu Wang PY - 2017/02 DA - 2017/02 TI - Expression of Beta-Adrenergic Receptor in Glioma LN229 Cells and Its Effect on Cell Proliferation BT - Proceedings of the 2016 7th International Conference on Education, Management, Computer and Medicine (EMCM 2016) PB - Atlantis Press SN - 2352-538X UR - https://doi.org/10.2991/emcm-16.2017.140 DO - https://doi.org/10.2991/emcm-16.2017.140 ID - Dong2017/02 ER -