Highly efficient and sensitive detection of chloramphenicol based on chemiluminescence immunoassays with functionalized Fe3O4@SiO2@Au magnetic nanoparticles
- DOI
- 10.2991/emcpe-16.2016.125How to use a DOI?
- Keywords
- chemiluminescent immunoassays; gold magnetic nanoparticles; chloramphenicol
- Abstract
To avoid the long time of dialysis process, a novel Strategy that Bovine serum albumin (BSA) was immobilized on Au-MNPs at first and afterward the CAP molecules were immobilized on the BSA in sequence has been applied to Chloramphenicol (CAP) detection with chemiluminescent immunoassays (CLIA) based on functionalized Fe3O4@SiO2@Au magnetic nanoparticles. More importantly, this designed method was successfully applied to CAP detection with the highly efficient and sensitive. With the functionalized gold magnetic nanoparticles (Au-MNPs). Samples were detected utilizing the two different chemiluminescence immunoassays, with limits of detection (LODs) of 0.07 pg mL-1 for Method I and 0.06 pg mL-1 for Method II and a 50% inhibition concentration (IC50) value of 0.086 ng mL-1 for Method I and 0.14 ng mL-1 for Method II for CAP respectively. Therefore, such chemiluminence immunoassays method could be easily adapted for small molecule detection in a variety of foods using Au-MNPs to conventional ILESA immunoassays in CAP detection.
- Copyright
- © 2016, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Linyu Wang AU - Manwen Yao AU - Xiangyi Fang AU - Zhuo Xu PY - 2016/08 DA - 2016/08 TI - Highly efficient and sensitive detection of chloramphenicol based on chemiluminescence immunoassays with functionalized Fe3O4@SiO2@Au magnetic nanoparticles BT - Proceedings of the 2016 5th International Conference on Environment, Materials, Chemistry and Power Electronics PB - Atlantis Press SP - 436 EP - 441 SN - 2352-5401 UR - https://doi.org/10.2991/emcpe-16.2016.125 DO - 10.2991/emcpe-16.2016.125 ID - Wang2016/08 ER -