Ability to Synthesize Extracellular Metabolites of Rice Endophytic Bacteria Before and After Gfp Gene Transformation
Ali Ikhwan, Fywit Eka Fransiska
Available Online November 2018.
- https://doi.org/10.2991/fanres-18.2018.35How to use a DOI?
- Endophytic Bacteria, Extracellular, Flourescent, Metabolite
- The purpose of this research was to investigate the ability of rice endophytic bacteria to synthesize extracellular metabolites before and after gfp (Green Fluorescent Protein) gene transformation. These bacteria were grown in liquid media M63+2mM Tryptophan for 3 days and the supernatants were extracted with absolute methanol 1:1 (v/v). The extractants were then analysed with Gas Chromatography-Mass Spectrophotometry (GC-MS). The GC-MS result showed that all isolates that were tested be it the control (without transformation) and the mutants (transformed) were able to synthesize growth hormones with different composition patterns, namely Indole 3 Butryric Acid (IBA), Indole Acetic Acid (IAA), Kinentin, Cis Zeatin, Zeatin, Benzylaminopurine, Giberellic Acid (GA3, GA1) as well as Zeatin Riboside. Furthermore, the isolates also synthesised osmoprotectants, namely Glycine, Proline, Arabinose, Thiamine, and Trehalose with different composition patterns as well
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Ali Ikhwan AU - Fywit Eka Fransiska PY - 2018/11 DA - 2018/11 TI - Ability to Synthesize Extracellular Metabolites of Rice Endophytic Bacteria Before and After Gfp Gene Transformation PB - Atlantis Press SP - 174 EP - 176 SN - 2352-5401 UR - https://doi.org/10.2991/fanres-18.2018.35 DO - https://doi.org/10.2991/fanres-18.2018.35 ID - Ikhwan2018/11 ER -