Proceedings of the International Conference on Food, Agriculture and Natural Resources (FANRes 2018)

Ability to Synthesize Extracellular Metabolites of Rice Endophytic Bacteria Before and After Gfp Gene Transformation

Authors
Ali Ikhwan, Fywit Eka Fransiska
Corresponding Author
Ali Ikhwan
Available Online November 2018.
DOI
https://doi.org/10.2991/fanres-18.2018.35How to use a DOI?
Keywords
Endophytic Bacteria, Extracellular, Flourescent, Metabolite
Abstract
The purpose of this research was to investigate the ability of rice endophytic bacteria to synthesize extracellular metabolites before and after gfp (Green Fluorescent Protein) gene transformation. These bacteria were grown in liquid media M63+2mM Tryptophan for 3 days and the supernatants were extracted with absolute methanol 1:1 (v/v). The extractants were then analysed with Gas Chromatography-Mass Spectrophotometry (GC-MS). The GC-MS result showed that all isolates that were tested be it the control (without transformation) and the mutants (transformed) were able to synthesize growth hormones with different composition patterns, namely Indole 3 Butryric Acid (IBA), Indole Acetic Acid (IAA), Kinentin, Cis Zeatin, Zeatin, Benzylaminopurine, Giberellic Acid (GA3, GA1) as well as Zeatin Riboside. Furthermore, the isolates also synthesised osmoprotectants, namely Glycine, Proline, Arabinose, Thiamine, and Trehalose with different composition patterns as well
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This is an open access article distributed under the CC BY-NC license.

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Proceedings
Part of series
Advances in Engineering Research
Publication Date
November 2018
ISBN
978-94-6252-619-8
ISSN
2352-5401
DOI
https://doi.org/10.2991/fanres-18.2018.35How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Ali Ikhwan
AU  - Fywit Eka Fransiska
PY  - 2018/11
DA  - 2018/11
TI  - Ability to Synthesize Extracellular Metabolites of Rice Endophytic Bacteria Before and After Gfp Gene Transformation
PB  - Atlantis Press
SP  - 174
EP  - 176
SN  - 2352-5401
UR  - https://doi.org/10.2991/fanres-18.2018.35
DO  - https://doi.org/10.2991/fanres-18.2018.35
ID  - Ikhwan2018/11
ER  -