Identification of Apoptosis Marker in Preeclampsia: A Pre-Eliminary Study of P53 Gene Expression in Human Placenta
- DOI
- 10.2991/aer.k.210825.038How to use a DOI?
- Keywords
- P53, Preeclampsia, Apoptosis
- Abstract
Preeclampsia still becomes a leading cause of maternal and perinatal mortality. The placenta is a critical subject in the pathogenesis of preeclampsia. Excessive apoptosis can cause the malfunction of trophoblast cells in their ability to remodel spiral arteries. Uteroplacental ischemia and endothelial cell injury may result in abnormal remodeling activity which may lead to preeclampsia-like symptoms. The p53 is a gene that acts as an apoptosis regulator in cells (as pro-apoptosis). This gene will cause the cascade apoptotic pathway, leading to cell death. Aim of this study to identify differences of p53 gene expression between preeclampsia and normal pregnancies. This is a case control study, performed in Biomolecular Laboratory Faculty of Medicine and Health science Universitas Jambi. Human placenta from patients with preeclampsia (n=25) and normal pregnancies as control (n=25) were collected from several hospitals in Jambi City from June 2020 to August 2020. Expression of p53 was assayed by quantitative real time PCR (qRT-PCR). Gene expression of p53 was significantly increased in pregnancies with preeclampsia (1,91± 5,90) compared to normal pregnancies group (4,85± 3,04) (t-test; p< 0.032). Expression of p53 has relatively increased by 7 fold for relative quantification by normalized with the internal control target gene GAPDH. Expression of p53 gene in preeclampsia is higher than normal pregnancies and There was significant difference between two groups in p53 gene expression.
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Herlambang Herlambang AU - Rina Nofri Enis AU - Susan Tarawifa AU - Tia Wida Ekaputri PY - 2021 DA - 2021/08/26 TI - Identification of Apoptosis Marker in Preeclampsia: A Pre-Eliminary Study of P53 Gene Expression in Human Placenta BT - Proceedings of the 3rd Green Development International Conference (GDIC 2020) PB - Atlantis Press SP - 209 EP - 213 SN - 2352-5401 UR - https://doi.org/10.2991/aer.k.210825.038 DO - 10.2991/aer.k.210825.038 ID - Herlambang2021 ER -