Toxicity Test of Muara Jambi Areca Nut (Areca Catechu L.) Extract on Rat Bone Marrow-derived Mesenchymal Stem Cells
- 10.2991/aer.k.210825.049How to use a DOI?
- osteoarthritis, areca nut, ethanol, alkaloids, flavonoids, MSC
Osteoarthritis (OA) is a joint inflammation process that happens when chondrocytes failure in maintaining the balance between degradation and extracellular matrix synthesis. Thus, it results in changes in the diameter and orientation of collagen fibers that change cartilage biomechanics and make joint cartilage lose its unique compressibility properties. This case is increasing every year globally, including Indonesia, but no drug could effectively treat this disease until recently. The use of areca nut as traditional anti-inflammatory drugs has long been known, but the scientific evidence is lacking. This study aims to develop a simple in vitro OA model that is useful for predicting the ability of drugs to reduce cartilage hypertrophy by using the seeds of areca nut (Areca catechu L). In this study, the extraction and fractionation of areca nut (Areca catechu L) were carried out to obtain the active substances in the form of ethanol, alkaloid, and flavonoid compounds. Acute toxicity assessment using the Methyl thiazole tetrazolium (MTT) method resulted in LD50 487.94 ppm. This shows that the Areca nut (Areca catechu L.) isolate is not toxic; therefore, it can be given as an alternative medicine for humans.
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Humaryanto AU - Esa Indah Ayudia AU - Muhaimin AU - Silmi Mariya PY - 2021 DA - 2021/08/26 TI - Toxicity Test of Muara Jambi Areca Nut (Areca Catechu L.) Extract on Rat Bone Marrow-derived Mesenchymal Stem Cells BT - Proceedings of the 3rd Green Development International Conference (GDIC 2020) PB - Atlantis Press SP - 273 EP - 280 SN - 2352-5401 UR - https://doi.org/10.2991/aer.k.210825.049 DO - 10.2991/aer.k.210825.049 ID - 2021 ER -