Clinical Assessment Using Disease Activity Index in Experimental Animal Model of Inflamatory Bowel Disease Induced Dextran Sulfate Sodium

DOI

10.2991/aer.k.210825.071How to use a DOI?

Keywords

Dextran sulfate sodium, Ulcerative colitis, Disease Activity Index

Abstract

Ulcerative colitis is an inflammation in the intestinal tract. Animal models of ulcerative colitis were used to study unknown pathogenesis and identify new drugs. Animal models of ulcerative colitis could be induced by chemical agents such as dextran sulfate sodium, trinitrobenzene sulfonic acid, and oxazolone. The colon inflammation induced by dextran sulfate sodium showed many clinical symptoms and immunologic reactions like in a human. This study aims to examine the animal model of ulcerative colitis induced by dextran sulfate sodium and assessing it with the Disease Activity Index. Nine male BALB/c mice 6-8 weeks, weigh 25-40 g divided into 3 groups. Group I (Control) was given drinking water only and group II induced by 2 cycles of 2% DSS for 5 days followed by drinking water for 10 days, and Group III induced by 3 cycles of 3% DSS for 7 days followed by drinking water for 7 days. The Admission of dextran sulfate sodium (Sigma-Aldrich code 42867, Mr~40,000) in drinking water was given ad libitum. The observed parameters for the Disease Activity Index were: body weight loss (score 0-4), feces consistency (score 0-4), and rectal bleeding (score 0-4). Feed consumption data is calculated by weighing the feed given minus the remaining feed (g/day). Drinking water consumption data is calculated by measuring the drinking water given minus the remaining drinking water (ml/day). In this study, mice in group II and group III which were induced with dextran sulfate sodium has no body weight loss, no diarrhea, and no rectal bleeding. The Disease Activity Index score was null. The feed consumption data for groups I, II, and III were 5.79 g/day, 6.58 g/day dan 5.21 g/day, respectively. The drinking water consumption data for groups I, II, and III were 5.97 ml/day, 5.70 ml/day, and 5.22 ml/day, respectively. Oral admission DSS 2% and DSS 3% did not establish clinical symptoms of ulcerative colitis in BALB/c mice. The research will be continued by looking at the colon of BALB/c mice macroscopically and microscopically.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Hanina Hanina
AU  - Tia Wida Ekaputri
AU  - Lipinwati
PY  - 2021
DA  - 2021/08/26
TI  - Clinical Assessment Using Disease Activity Index in Experimental Animal Model of Inflamatory Bowel Disease Induced Dextran Sulfate Sodium
BT  - Proceedings of the 3rd Green Development International Conference (GDIC 2020)
PB  - Atlantis Press
SP  - 416
EP  - 420
SN  - 2352-5401
UR  - https://doi.org/10.2991/aer.k.210825.071
DO  - 10.2991/aer.k.210825.071
ID  - Hanina2021
ER  -