Proceedings of the 2nd International Conference on Green Materials and Environmental Engineering

Community Structure and Quantification of Nitrifying Bacteria in Integrated Fixed Film Activated Sludge System Treating Industrial Wastewater

Authors
Chao Li, Weili Xi, Tao Bi
Corresponding Author
Chao Li
Available Online December 2015.
DOI
https://doi.org/10.2991/gmee-15.2015.19How to use a DOI?
Keywords
IFAS; AOB; NOB; PCR-DGGE; real time PCR
Abstract

To reveal the community structure and abundance of ammonia-oxidizing bacterial (AOB) and nitrite-oxidizing bacterial (NOB) in integrated fixed film activated sludge process (IFAS) treating industrial wastewater, the molecular biological techniques of polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), cloning and sequencing and real-time quantitative PCR were applied. DGGE results showed that in the process of the experiment, the dominant species of suspended sludge and biofilm were different and that some kinds of populations had been highly preponderance consistently. Additionally, variation in influents had no significant effect on the distribution of the AOB and NOB communities. Sequencing analysis of screened clones revealed Nitrosospira sp. was the particular AOB population in suspended sludge, and some members of Nitrosomonas sp. are helpful to keep the biomass remained in the bio-carrier surface. Real-time PCR indicated that the proportion of the AOB and NOB in total amount of bacteria increased significantly after the domestication period. The AOB and NOB population in the suspended sludge were even higher than the content of the biofilm.

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2nd International Conference on Green Materials and Environmental Engineering
Series
Advances in Engineering Research
Publication Date
December 2015
ISBN
978-94-6252-138-4
ISSN
2352-5401
DOI
https://doi.org/10.2991/gmee-15.2015.19How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Chao Li
AU  - Weili Xi
AU  - Tao Bi
PY  - 2015/12
DA  - 2015/12
TI  - Community Structure and Quantification of Nitrifying Bacteria in Integrated Fixed Film Activated Sludge System Treating Industrial Wastewater
BT  - Proceedings of the 2nd International Conference on Green Materials and Environmental Engineering
PB  - Atlantis Press
SP  - 66
EP  - 69
SN  - 2352-5401
UR  - https://doi.org/10.2991/gmee-15.2015.19
DO  - https://doi.org/10.2991/gmee-15.2015.19
ID  - Li2015/12
ER  -