Proceedings of the 2013 International Academic Workshop on Social Science

The Fluorescence Characteristic of AO-RB-SDS-GMFX and the Determination of Gemifloxacin

Authors
Hui-ping Xi, Xiu-wei Fang
Corresponding Author
Hui-ping Xi
Available Online October 2013.
DOI
https://doi.org/10.2991/iaw-sc.2013.145How to use a DOI?
Keywords
Gemifloxacin; fluorescence resonance energy transfer; fluorescence quenching; Acridine Orange; Rhodamine B
Abstract
A new method for the determination of Gemifloxacin (GMFX) indirectly was developed by using energy transfer of Acridine Orange (AO) - Rhodamine B (RB) fluorescence resonance. It was found that in the pH=7.00 Britton-Robinson (B-R) buffer solution and sodium dodecyl sulfate (SDS) medium, effective energy transfer could occur between AO and RB the fluorescence of RB was enhanced. The fluorescence intensity of RB quenched with the addition of the GMFX. Using this method to determine the GMFX tablet, the results were consistent with high performance liquid chromatography (HPLC) method, the sample recovery was 98.58~102.16%, the relative standard deviation (RSD) was 0.68 ~2.2%, the detection limit of this method was 0.025mg • L-1. Experiments showed that this method was simple, rapid, accurate, and sensitive.
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Proceedings
International Academic Workshop on Social Science (IAW-SC-13)
Part of series
Advances in Intelligent Systems Research
Publication Date
October 2013
ISBN
978-90-78677-84-0
ISSN
1951-6851
DOI
https://doi.org/10.2991/iaw-sc.2013.145How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Hui-ping Xi
AU  - Xiu-wei Fang
PY  - 2013/10
DA  - 2013/10
TI  - The Fluorescence Characteristic of AO-RB-SDS-GMFX and the Determination of Gemifloxacin
BT  - International Academic Workshop on Social Science (IAW-SC-13)
PB  - Atlantis Press
SN  - 1951-6851
UR  - https://doi.org/10.2991/iaw-sc.2013.145
DO  - https://doi.org/10.2991/iaw-sc.2013.145
ID  - Xi2013/10
ER  -