The Fluorescence Characteristic of AO-RB-SDS-GMFX and the Determination of Gemifloxacin
- 10.2991/iaw-sc.2013.145How to use a DOI?
- Gemifloxacin; fluorescence resonance energy transfer; fluorescence quenching; Acridine Orange; Rhodamine B
A new method for the determination of Gemifloxacin (GMFX) indirectly was developed by using energy transfer of Acridine Orange (AO) - Rhodamine B (RB) fluorescence resonance. It was found that in the pH=7.00 Britton-Robinson (B-R) buffer solution and sodium dodecyl sulfate (SDS) medium, effective energy transfer could occur between AO and RB the fluorescence of RB was enhanced. The fluorescence intensity of RB quenched with the addition of the GMFX. Using this method to determine the GMFX tablet, the results were consistent with high performance liquid chromatography (HPLC) method, the sample recovery was 98.58~102.16%, the relative standard deviation (RSD) was 0.68 ~2.2%, the detection limit of this method was 0.025mg • L-1. Experiments showed that this method was simple, rapid, accurate, and sensitive.
- © 2013, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Hui-ping Xi AU - Xiu-wei Fang PY - 2013/10 DA - 2013/10 TI - The Fluorescence Characteristic of AO-RB-SDS-GMFX and the Determination of Gemifloxacin BT - Proceedings of the 2013 International Academic Workshop on Social Science PB - Atlantis Press SP - 657 EP - 660 SN - 1951-6851 UR - https://doi.org/10.2991/iaw-sc.2013.145 DO - 10.2991/iaw-sc.2013.145 ID - Xi2013/10 ER -