Determination of acrylamide in roasted coffee by UPLC-MS/MS

DOI

10.2991/ic3me-15.2015.73How to use a DOI?

Keywords

Ultra-high performance liquid chromatography-mass spectrometry; Acrylamide; Roasted coffee

Abstract

A method for the determination of acrylamide in roasted coffee by UPLC-MS/MS was established. Acrylamide was extracted with water, and clean up on a HLB and MCX SPE cartridge, separated on a C18 column using acetonitrile-0.1% formic acid as mobile phase, and then detected under multiple reaction monitoring mode Deuterium labelled D3-acrylamide was used as internal standard. The recoveries ranged from 87.2 to 92.0 at three spiked concentrations with the relative standard deviations lower than 6 . The LOD and LOQ were 5 and 15 g/kg, respectively.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Shaodong Zeng
AU  - Tongyu Xu
AU  - Mingyue Wang
AU  - Chunliang Yang
PY  - 2015/08
DA  - 2015/08
TI  - Determination of acrylamide in roasted coffee by UPLC-MS/MS
BT  - Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering
PB  - Atlantis Press
SP  - 376
EP  - 379
SN  - 2352-5401
UR  - https://doi.org/10.2991/ic3me-15.2015.73
DO  - 10.2991/ic3me-15.2015.73
ID  - Zeng2015/08
ER  -