Enrichment and purification of phenols from Callicarpa nudiflora Hook. et Arn by HP-20 macroporous resin
- DOI
- 10.2991/ic3me-15.2015.81How to use a DOI?
- Keywords
- C. nudiflora; phenols; HP-20 macroporous resin
- Abstract
Objective To study the technological parameters of the purification process of phenols from Callicarpa nudiflora (C. nudiflora) with HP-20 macroporous resin. Methods The kinetic adsorption and desorption experiments were carried out on HP-20 macroporous resin (HP-20) to optimize the separation process of phenols. Additionally, the effects of four parameters including adsorption flow rate, elute flow rate, volume of 10% ethanol and 50% ethanol solution for elution were explored by a L4/3 orthogonal experiment. Finally, content of phenols in samples before and after being treated by HP-20 were compared. Results The results showed that a good separation and purification of crude total phenols extract from C. nudiflora was achieved using HP-20. The optimum conditions were found to be: initial concentration of 50 mg mL-1, absorb flow rate of 1 mL min-1, elute flow rate of 3 mL min-1, 5 BV of 10% ethanol solution for prewash and 10 BV of 50% ethanol solution for desorption solvent. Conclusion The content of phenols is above 40% in C. nudiflora after being treated by HP-20, indicating that HP-20 could be successfully applied to enrich and purify phenols in C. nudiflora.
- Copyright
- © 2015, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Xiaojie Yan AU - Sheng Huang AU - Fenglai Lu AU - Zhixin Gu AU - Lei Wang AU - Yonglin Huang AU - Dianpeng Li PY - 2015/08 DA - 2015/08 TI - Enrichment and purification of phenols from Callicarpa nudiflora Hook. et Arn by HP-20 macroporous resin BT - Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering PB - Atlantis Press SP - 412 EP - 416 SN - 2352-5401 UR - https://doi.org/10.2991/ic3me-15.2015.81 DO - 10.2991/ic3me-15.2015.81 ID - Yan2015/08 ER -