Prepared polyclonal antibody of the semicarbazide

DOI

10.2991/ic3me-15.2015.90How to use a DOI?

Keywords

Nitrofurazone ;Semicarbazide; Hapten; Conjuguate; Polyclonal Antibody

Abstract

Nitrofurazone residues in fishery products were dangerous for human health , semicarbazide (SEM) was the nitrofurazone metabolite could residues in fishery in a long time . To detect SEM residues in fishery products rapidly by immune method, preparation of high quality polyclonal antibody against SEM was the most critical step of project. We produced two kinds of antigens for SEM, using carrier protein bovine serum albumin (BSA) and ovalbumin (OVA) by a modified carbodiimide method, and then the hapten-protein conjugates were characterized by ultraviolet spectrophotometry in detecting qualitatively comparable hapten density, before being used for the immunization and detection purposes. The production of polyclonal antibodies (PcAbs) were sought following the generation of appropriate SEM-BSA conjugate. One PcAb against Semicarbazide (SEM) was produced and the titer polyclonal antibody could reach 105. In the optimized ELISA, the PcAb showed 50% inhibition at 25.0ng/mL for SEM in buffer.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Xuan-Yun Huang
AU  - Dong-Mei Huang
AU  - You-Qiong Cai
PY  - 2015/08
DA  - 2015/08
TI  - Prepared polyclonal antibody of the semicarbazide
BT  - Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering
PB  - Atlantis Press
SP  - 458
EP  - 461
SN  - 2352-5401
UR  - https://doi.org/10.2991/ic3me-15.2015.90
DO  - 10.2991/ic3me-15.2015.90
ID  - Huang2015/08
ER  -