Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering

Prepared polyclonal antibody of the semicarbazide

Authors
Xuan-Yun Huang, Dong-Mei Huang, You-Qiong Cai
Corresponding Author
Xuan-Yun Huang
Available Online August 2015.
DOI
https://doi.org/10.2991/ic3me-15.2015.90How to use a DOI?
Keywords
Nitrofurazone ;Semicarbazide; Hapten; Conjuguate; Polyclonal Antibody
Abstract
Nitrofurazone residues in fishery products were dangerous for human health , semicarbazide (SEM) was the nitrofurazone metabolite could residues in fishery in a long time . To detect SEM residues in fishery products rapidly by immune method, preparation of high quality polyclonal antibody against SEM was the most critical step of project. We produced two kinds of antigens for SEM, using carrier protein bovine serum albumin (BSA) and ovalbumin (OVA) by a modified carbodiimide method, and then the hapten-protein conjugates were characterized by ultraviolet spectrophotometry in detecting qualitatively comparable hapten density, before being used for the immunization and detection purposes. The production of polyclonal antibodies (PcAbs) were sought following the generation of appropriate SEM-BSA conjugate. One PcAb against Semicarbazide (SEM) was produced and the titer polyclonal antibody could reach 105. In the optimized ELISA, the PcAb showed 50% inhibition at 25.0ng/mL for SEM in buffer.
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This is an open access article distributed under the CC BY-NC license.

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Proceedings
3rd International Conference on Material, Mechanical and Manufacturing Engineering (IC3ME 2015)
Part of series
Advances in Engineering Research
Publication Date
August 2015
ISBN
978-94-6252-100-1
ISSN
2352-5401
DOI
https://doi.org/10.2991/ic3me-15.2015.90How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Xuan-Yun Huang
AU  - Dong-Mei Huang
AU  - You-Qiong Cai
PY  - 2015/08
DA  - 2015/08
TI  - Prepared polyclonal antibody of the semicarbazide
BT  - 3rd International Conference on Material, Mechanical and Manufacturing Engineering (IC3ME 2015)
PB  - Atlantis Press
SN  - 2352-5401
UR  - https://doi.org/10.2991/ic3me-15.2015.90
DO  - https://doi.org/10.2991/ic3me-15.2015.90
ID  - Huang2015/08
ER  -