Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering

Highly sensitive fluorescent aptasensor for adenosine detection based on gold nanoparticle tagged molecular beacon

Authors
Liying Zheng, Songbai Zhang, Jinlin Lu, Xia Hu, Guangyu Shen, Xuewen Liu, Yuandao Chen, Liping Qiu, Shibiao Zhou
Corresponding Author
Liying Zheng
Available Online October 2015.
DOI
https://doi.org/10.2991/icadme-15.2015.57How to use a DOI?
Keywords
Molecular beacon, aptasensor, gold nanoparticle, fluorescent, adenosine
Abstract
In this contribution, a sensitive fluorescent aptasensing strategy for the detection of adenosine is proposed based on structure switching of gold nanoparticle modified molecular beacon. Hairpin structured molecular beacon exhibits low background fluorescence since the fluorophore and nanoparticle quencher are in close proximity in the absence of adenosine. Binding with target molecules induces structure switching of molecular beacon, resulting in significant fluorescence increase due to separation of fluorophore and quencher. The fluorescence intensity is linear with adenosine concentration in a dynamic range from 90nM -1.5 M with a detection limit of 60 nM. Moreover, good reproducibility and high specificity are achieved.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Volume Title
Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering
Series
Advances in Engineering Research
Publication Date
October 2015
ISBN
978-94-6252-113-1
ISSN
2352-5401
DOI
https://doi.org/10.2991/icadme-15.2015.57How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Liying Zheng
AU  - Songbai Zhang
AU  - Jinlin Lu
AU  - Xia Hu
AU  - Guangyu Shen
AU  - Xuewen Liu
AU  - Yuandao Chen
AU  - Liping Qiu
AU  - Shibiao Zhou
PY  - 2015/10
DA  - 2015/10
TI  - Highly sensitive fluorescent aptasensor for adenosine detection based on gold nanoparticle tagged molecular beacon
BT  - Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering
PB  - Atlantis Press
SP  - 287
EP  - 290
SN  - 2352-5401
UR  - https://doi.org/10.2991/icadme-15.2015.57
DO  - https://doi.org/10.2991/icadme-15.2015.57
ID  - Zheng2015/10
ER  -