Improvement of two-dimensional gel electrophoresis conditions for rin mutant tomato fruit

DOI

10.2991/icaees-15.2015.232How to use a DOI?

Keywords

mutant rin, proteomics, two-dimensional gel electrophoresis, optimum condition

Abstract

Tomato fruit contains a lot of pigment, polysaccharide and secondary metabolism substances. These substances can affect protein separation effect, and interfere with the process of two-dimensional gel electrophoresis. The extraction of tomato fruit protein sample, conditions of isoelectric focusing and two-dimensional gel electrophoresis had been investigated to establish a suitable method of rin mutant tomato fruit. It was shown that the TCA-acetone precipitation combined with clean-up kit was the best method for tomato fruit protein extraction. The best condition was pH 4-7 IPG, lysis buffer , 500 g sample. The 2-DE protocol suited for the separation of proteins from rin mutant tomato fruit was established.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Ling Li
AU  - Yuanyuan Song
AU  - Yifeng Wang
AU  - Baoxin Zheng
AU  - Tieling Liu
PY  - 2015/07
DA  - 2015/07
TI  - Improvement of two-dimensional gel electrophoresis conditions for rin mutant tomato fruit
BT  - Proceedings of the 3rd International Conference on Advances in Energy and Environmental Science 2015
PB  - Atlantis Press
SP  - 1249
EP  - 1253
SN  - 2352-5401
UR  - https://doi.org/10.2991/icaees-15.2015.232
DO  - 10.2991/icaees-15.2015.232
ID  - Li2015/07
ER  -