Proceedings of the 7th International Conference on Biological Science (ICBS 2021)

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CO2 Mitochondrial Gene Identification of Nisaetus cirrhatus as a Part of Indonesian Elang Brontok Genetic Conservation

Authors
Reni Krisdayana1, Rizqi Layli Khusufi1, Dwita Novitasari1, Annasa Sabatia1, Delia Wahyu Pangesti1, Salsabila Kasta Hygiea Iswara2, Dwi Listyorini1, 2, *
1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang
2Department of Biotechnology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang No.5, Malang, 65145, Indonesia
*Corresponding author.Email: listyorini.aljabari@um.ac.id
Corresponding Author
Dwi Listyorini
Available Online 2 May 2022.
DOI
10.2991/absr.k.220406.047How to use a DOI?
Keywords
Genetic conservation; Indonesia; MT-COI; MT-CO2; Nisaetus cirrhatus
Abstract

The Elang Brontok, scientifically named as Nisaetus cirrhatus is included in the least Concern in appendix II. The use of the COI gene for taxonomic in our previous study put our samples in a complex species taxonomical position. Further research needs to be done using other mitochondrial genes such as CO2. In addition, the CO2 sequence of Nisaetus cirrhatus has not yet been reported. The purpose of this study was to obtain complete sequences of COI and CO2 and determine the taxonomic position of the Elang Brontok (Nisaetus cirrhatus) in Indonesia. In this study, we used the same three samples as of the previous study for CO1. We designed a pair of primers based on the previously found CO1 sequence with not yet known the length of the target gene since there are no reports for it. Phylogenetic tree reconstruction proved that the Elang Brontok we studied belongs to Nisaetus cirrhatus. The result of combining sequences obtained from this study with one previously reported showed some mutations at the 5’ end of COI. We suggest that the extended 3’ end of COI is part of the yet reported Nisaetus cirrhatus CO2 sequence.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
Series
Advances in Biological Sciences Research
Publication Date
2 May 2022
ISBN
10.2991/absr.k.220406.047
ISSN
2468-5747
DOI
10.2991/absr.k.220406.047How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

risenwbib
TY  - CONF
AU  - Reni Krisdayana
AU  - Rizqi Layli Khusufi
AU  - Dwita Novitasari
AU  - Annasa Sabatia
AU  - Delia Wahyu Pangesti
AU  - Salsabila Kasta Hygiea Iswara
AU  - Dwi Listyorini
PY  - 2022
DA  - 2022/05/02
TI  - CO2 Mitochondrial Gene Identification of Nisaetus cirrhatus as a Part of Indonesian Elang Brontok Genetic Conservation
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 331
EP  - 337
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.047
DO  - 10.2991/absr.k.220406.047
ID  - Krisdayana2022
ER  -