Biochemical Characterization of Acetamiprid Resistance in Laboratory-Bred Population of Aedes aegypti L. Larvae

DOI

10.2991/absr.k.200513.030How to use a DOI?

Keywords

Aedes aegypti, acetamiprid, esterases, glutathione-S-transferase, acetylcholinesterases

Abstract

The constant rise in cases of Zika, Dengue and Chikungunya worldwide has made control of Aedes aegypti a principal concern. The most recommended plan to control mosquito-borne diseases primarily lies on vector management and disturbing their disease-transmission cycle. Wide-ranging use of different classes of organic insecticides for mosquito control has led to the development of high levels of resistance making them less operative at safe dosages imposing us to explore novel insecticides. Present study investigates the bio-efficacy of a neonicotinoid, acetamiprid on the Ae. aegypti larvae, development of resistance after subjecting acetamiprid selection pressure for 10 successive generations and biochemical characterization of the resistance developed. Acetamiprid exposure of the parent population of Ae. aegypti early fourth instars resulted in respective LC50 and LC90 values of 0.188 ppm and 1.315 ppm. Selection with acetamiprid for 10 successive generations (ACSF-10) reduced its efficacy by 20-fold. Involvement of four enzymes; alpha-esterases, beta-esterases, glutathione-S-transferases and acetylcholinesterases in development of acetamiprid resistance was investigated to uncover mode of action of acetamiprid. An elevation of 1.4-fold and 2.1-fold was observed in alpha-esterases and beta-esterases activity in ACSF-10 as compared to ACSF-5. However, activity of glutathione-S-transferases decreased in ACSF-5 which rose to 12-fold in ACSF-10. Similarly, the activity of acetylcholinesterases was found to be much higher in resistant generations as compared to the parental strains. The results indicated individual/synergistic contribution of different enzymes leading to acetamiprid detoxification. Further research is being conducted to identify the role of target site mutations in resistance development.

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Roopa Rani Samal
AU  - Kungreiliu Panmei
AU  - P Lanbiliu
AU  - Sarita Kumar
PY  - 2020
DA  - 2020/05/18
TI  - Biochemical Characterization of Acetamiprid Resistance in Laboratory-Bred Population of Aedes aegypti L. Larvae
BT  - Proceedings of the International Conference and the 10th Congress of the Entomological Society of Indonesia (ICCESI 2019)
PB  - Atlantis Press
SP  - 169
EP  - 176
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.200513.030
DO  - 10.2991/absr.k.200513.030
ID  - Samal2020
ER  -