Extraction, purification and identification of the allergen from fresh Amaranthus stamen
- DOI
- 10.2991/iccte-16.2016.165How to use a DOI?
- Keywords
- Amaranthus stamen; Allergen; Analysis
- Abstract
Objective:To purify and characterize the allergen extracted from fresh Amaranthus stamen. Methods:Extraction of fresh Amaranthus stamen was prepared and analyzed by SDS-PAGE. The protein bands were visualized by staining the gel with Coomassis blue and analyzed by Western-blotting with serum from allergic patients with positive skin test of Amaranthus pollen. Then, FPLC and Ion-exchange chromatography with DE-52 was used for purification of the antigens and the purified products were characterized by Western-blotting. Results: Twenty protein bands were detected in SDS-PAGE. Four of these bands with molecular weight of 66, 36, 30, and 18 kDa showed immumo-reactivity with IgE in the sera from patients with allergy to Amaranthus pollen. The 66, 36, 30, and18 kDa protein was the major allergen component. Meanwhile, the protein from the Amaranthus stamen were distributed in different specimen respectively collectted according to peak - . It was indicated that the major allergens from the Amaranthus stamen were eluted in specimen corresponding to the peak 66,30 kDa , - (36 kDa) and 18kDa by the Ion-exchange chromatography and Western-blotting. Conclusion: Extraction from Amaranthus stamen was initially isolated , purified and characterized. The major allergens was 66, 36, 30, and 18 kDa protein.
- Copyright
- © 2016, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Shuixiang Xie AU - Lichun Wen AU - Xiang Gong AU - Xiao Jun Xiao AU - Li Ma AU - Zhigang Liu PY - 2016/01 DA - 2016/01 TI - Extraction, purification and identification of the allergen from fresh Amaranthus stamen BT - Proceedings of the 2016 International Conference on Civil, Transportation and Environment PB - Atlantis Press SP - 950 EP - 955 SN - 2352-5401 UR - https://doi.org/10.2991/iccte-16.2016.165 DO - 10.2991/iccte-16.2016.165 ID - Xie2016/01 ER -