The Activity of Anti-Oxidide of Etil Acetate Soluble Fractions from Extract of Kedayan Roots (Aristolochia Papillfolia Ding Hou)

DOI

10.2991/icedutech-17.2018.19How to use a DOI?

Keywords

Kedayan roots (Aristolochia papillfolia Ding hou); antioxidant; IC50;

Abstract

Kedayan roots (Aristolochia papillfolia Ding hou) is phytomedicine that used conventionally by peoples as antidotum. This research aims to know antioxidant activity of ethyl acetate soluble fraction from kedayan roots extract. Fractionation method that used is solid-liquid extraction with ethyl acetat as solven so that produced fraction that dissolve in ethyl acetate, than fractionated again with liquid vacuum chromatography with eluent n-hexane and ethyl acetat with some proportion of each solven and produced fraction A (5:5), fraction B (4:5), fraction C (3:5), fraction D (2:5), fraction E (1:5), and fraction F (0:5). Antioxidant activity assay used DPPH (2,2-diphenyl-1-picrylhydrazyl) as radical substance and determined by visible spectrophotometre. Than data analyzed with linier regression to know IC50. The result of fraction C from kedayan roots (Aristolochia pap illfolia Ding hou) in ethyl acetate fraction have antioxidant activity with IC50 139,11 ppm.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Novermawati Novermawati
AU  - Muh. Amir
AU  - Islamudin Islamudin
PY  - 2017/11
DA  - 2017/11
TI  - The Activity of Anti-Oxidide of Etil Acetate Soluble Fractions from Extract of Kedayan Roots (Aristolochia Papillfolia Ding Hou)
BT  - Proceedings of the 2017 International Conference on Education and Technology (2017 ICEduTech)
PB  - Atlantis Press
SP  - 100
EP  - 102
SN  - 1951-6851
UR  - https://doi.org/10.2991/icedutech-17.2018.19
DO  - 10.2991/icedutech-17.2018.19
ID  - Novermawati2017/11
ER  -