Proceedings of the 2018 7th International Conference on Energy, Environment and Sustainable Development (ICEESD 2018)

Effect of different terminators on transcription regulatory factor ClrB and XlnR in Penicillium oxalicum 114-2

Authors
Qin Yan, Yanan Wang, Xiaoming Bao, Yinbo Qu, XinLi Liu, Zhonghai Li
Corresponding Author
Qin Yan
Available Online May 2018.
DOI
https://doi.org/10.2991/iceesd-18.2018.225How to use a DOI?
Keywords
Lignocellulose Cellulase and hemicellulase Terminators ClrB and XlnR
Abstract

ClrB and XlnR positively regulated cellulase and hemicellulase expression in Penicillium oxalicum 114-2. In addition,terminators, as promoters, play an important role in gene transcription. T- his research mainly investigated the effects of TbglII, TcbhI, TegI, TD, TgpdA on the regulatory fact- ors ClrB and XlnR. By replacing TclrB and TxlnR with TbglII, TcbhI, TegI, TD, TgpdA, we explored the effect of different terminators on two regulatory factors and ultimately achieved the purpose of improving cellulase activity. The results showed that the terminator had a significant influence on the regulatory function of regulators, and the FPA, CMC, pNPX and pNPC enzyme activity of the recom- binant strains obtained were significantly higher than that of wild-type 114-2 and the highest FPA enzyme activity reached 1.4 U/mL (c-d15 -2-1). These were clearly not enough compared to high-yield cellulases strains, but here provided new insight into the improving the cellulase and hemicellulase activity. Terminators had a huge potential for the production of cellulase and hemicellulase.

Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2018 7th International Conference on Energy, Environment and Sustainable Development (ICEESD 2018)
Series
Advances in Engineering Research
Publication Date
May 2018
ISBN
978-94-6252-503-0
ISSN
2352-5401
DOI
https://doi.org/10.2991/iceesd-18.2018.225How to use a DOI?
Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Qin Yan
AU  - Yanan Wang
AU  - Xiaoming Bao
AU  - Yinbo Qu
AU  - XinLi Liu
AU  - Zhonghai Li
PY  - 2018/05
DA  - 2018/05
TI  - Effect of different terminators on transcription regulatory factor ClrB and XlnR in Penicillium oxalicum 114-2
BT  - Proceedings of the 2018 7th International Conference on Energy, Environment and Sustainable Development (ICEESD 2018)
PB  - Atlantis Press
SP  - 1220
EP  - 1226
SN  - 2352-5401
UR  - https://doi.org/10.2991/iceesd-18.2018.225
DO  - https://doi.org/10.2991/iceesd-18.2018.225
ID  - Yan2018/05
ER  -