Effect of different terminators on transcription regulatory factor ClrB and XlnR in Penicillium oxalicum 114-2
- 10.2991/iceesd-18.2018.225How to use a DOI?
- Lignocellulose Cellulase and hemicellulase Terminators ClrB and XlnR
ClrB and XlnR positively regulated cellulase and hemicellulase expression in Penicillium oxalicum 114-2. In addition,terminators, as promoters, play an important role in gene transcription. T- his research mainly investigated the effects of TbglII, TcbhI, TegI, TD, TgpdA on the regulatory fact- ors ClrB and XlnR. By replacing TclrB and TxlnR with TbglII, TcbhI, TegI, TD, TgpdA, we explored the effect of different terminators on two regulatory factors and ultimately achieved the purpose of improving cellulase activity. The results showed that the terminator had a significant influence on the regulatory function of regulators, and the FPA, CMC, pNPX and pNPC enzyme activity of the recom- binant strains obtained were significantly higher than that of wild-type 114-2 and the highest FPA enzyme activity reached 1.4 U/mL (c-d15 -2-1). These were clearly not enough compared to high-yield cellulases strains, but here provided new insight into the improving the cellulase and hemicellulase activity. Terminators had a huge potential for the production of cellulase and hemicellulase.
- © 2018, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Qin Yan AU - Yanan Wang AU - Xiaoming Bao AU - Yinbo Qu AU - XinLi Liu AU - Zhonghai Li PY - 2018/05 DA - 2018/05 TI - Effect of different terminators on transcription regulatory factor ClrB and XlnR in Penicillium oxalicum 114-2 BT - Proceedings of the 2018 7th International Conference on Energy, Environment and Sustainable Development (ICEESD 2018) PB - Atlantis Press SP - 1220 EP - 1226 SN - 2352-5401 UR - https://doi.org/10.2991/iceesd-18.2018.225 DO - 10.2991/iceesd-18.2018.225 ID - Yan2018/05 ER -