Proceedings of the 3rd International Conference on Environmentally Sustainable Animal Industry 2022 (ICESAI 2022)

The Use of Kefir Grains as an Anaerobic Method for Removal of β-Lactam Antibiotic Residue on Cows Milk

Authors
Ahmad Khoirul Umam1, Lilik Eka Radiati1, *, Ria Dewi Andriani1, Citra Nurma Yunita1, Millenia Wahyu Ramadhaniarti1, Rizky Ramadhan1
1Animal Product Technology Department, Faculty of Animal Science, Brawijaya University, Malang, Indonesia
*Corresponding author. Email: lilik.eka@ub.ac.id
Corresponding Author
Lilik Eka Radiati
Available Online 8 March 2023.
DOI
10.2991/978-94-6463-116-6_30How to use a DOI?
Keywords
antibiotic; milk; kefir grain
Abstract

After mastitis therapy, using the right technology, it was necessary to break down the antibiotics in waste milk. Due to microbial activity, the microbiology approach as an anaerobic treatment presumably successfully removes antibiotic residues in milk. This study attempted to Eliminate Antibiotic Residue from Mastitis Treatment in Cow’s Milk by Using Kefir Grain in an Anaerobic Method. Twelve samples of Holstein cows milk with 3% kefir grain were randomly assigned to one of four treatments: cows milk with 3% kefir grain and no antibiotics (control), cow milk with 3% kefir grain and six, eight, and ten micrograms per milliliter of Amoxycillin trihydrate, respectively. Completely Randomized Design (CRD) with four treatments and three replications was used to evaluate the collected data, followed by Duncan’s Multiple Range Test (DMRT). This study demonstrates that adding 3% kefir grains eliminates antibiotics in milk, as indicated by a negative antibiotic residue test. On cow’s milk containing 3% kefir grain and 6 μg/ml, a significantly different effect of pH (3.98 ± 0.2), syneresis (24.93 ± 0.7), and lactic acid bacteria viability (8.88 CFU/ml) were observed, except for dissolved protein (8.16 ± 1.7).

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 3rd International Conference on Environmentally Sustainable Animal Industry 2022 (ICESAI 2022)
Series
Advances in Biological Sciences Research
Publication Date
8 March 2023
ISBN
10.2991/978-94-6463-116-6_30
ISSN
2468-5747
DOI
10.2991/978-94-6463-116-6_30How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

TY  - CONF
AU  - Ahmad Khoirul Umam
AU  - Lilik Eka Radiati
AU  - Ria Dewi Andriani
AU  - Citra Nurma Yunita
AU  - Millenia Wahyu Ramadhaniarti
AU  - Rizky Ramadhan
PY  - 2023
DA  - 2023/03/08
TI  - The Use of Kefir Grains as an Anaerobic Method for Removal of β-Lactam Antibiotic Residue on Cows Milk
BT  - Proceedings of the 3rd International Conference on Environmentally Sustainable Animal Industry 2022 (ICESAI 2022)
PB  - Atlantis Press
SP  - 233
EP  - 239
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-116-6_30
DO  - 10.2991/978-94-6463-116-6_30
ID  - Umam2023
ER  -