Detection of Leptospira Bacteria in Rats in Ciomas Sub-district, Bogor District, West Jawa Using Real-Time PCR Method

DOI

10.2991/978-94-6463-112-8_86How to use a DOI?

Keywords

Leptospira; Rats; PCR

Abstract

Leptospirosis is a zoonotic disease caused by bacteria from the genus Leptospira. Leptospira bacteria can infect rats, dogs, cats, raccoons, cattle, horses, and other mammals. The main reservoir of leptospirosis in Indonesia is rats. Indonesia is a country with a high risk of developing leptospirosis, considering the occurrence of flooding and the presence of stagnant water, and poor drainage and sanitation conditions in several residential areas. This study aims to detect Leptospira spp. bacteria in kidney tissues of rats in the Ciomas Sub-district, Bogor District, West Java. This study was a descriptive-analytic study with a cross-sectional design. Its survey was conducted in Batu City Village, Mekarjaya, and Parakan, Ciomas Sub-district. The rats caught were counted for density, identified the species, and taken kidney samples to be examined for the presence of Leptospira using the Real-Time Polymerase Chain Reaction (RT-PCR) method. The density of rats in this study, which was based on the success of catching rats in Parakan Village, was 26%, Mekarjaya 17.3%, and Kota Batu 15.3%. The total number of rats caught was 88. The rat species caught were Rattus tanezumi (54.5%), Bandicota indica (21.6%), Rattus norvegicus (20.5%) and Mus musculus (3.4%). The results of the PCR test found that 22 out of 88 rat samples were positive for Leptospira. All species caught were positive for Leptospira with the following percentages, Bandicota indica (50%), Rattus norvegicus (27.3%), Rattus tanezumi (18.2%), and Mus musculus (4.5%). Leptospira bacteria were successfully detected in rat kidneys using the RT- PCR method. The public can increase their awareness by keeping the environment clean in an effort to prevent leptospirosis transmission.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Gunawan Gunawan
AU  - Hanny Zamsiar
AU  - Agus A. Wibowo
AU  - Yahidin Selian
AU  - Dessy L. Utami
PY  - 2023
DA  - 2023/03/01
TI  - Detection of Leptospira Bacteria in Rats in Ciomas Sub-district, Bogor District, West Jawa Using Real-Time PCR Method
BT  - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
PB  - Atlantis Press
SP  - 949
EP  - 956
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-112-8_86
DO  - 10.2991/978-94-6463-112-8_86
ID  - Gunawan2023
ER  -