Identification of Antioxidant Active Compounds from Watercress (Nasturtium officinale R.Br)
- DOI
- 10.2991/978-94-6463-112-8_35How to use a DOI?
- Keywords
- antioxidant; DPPH; Nasturtium officinale R.Br; phenol
- Abstract
Watercress (Nasturtium officinale R.Br) is a highlands plant that has been eaten as a vegetable by many people. Watercress contains protein, calcium, phosphorus, iron, vitamin A, vitamin E, vitamin C, flavonoids and phenols. Antioxidants are components that can delay or prevent the oxidation of lipids, nucleic acids, or other molecules by inhibiting the initiation or propagation of oxidation chain reactions. The function of antioxidants is to neutralize free radicals, so that the body is protected from various degenerative diseases and helps suppress the aging process. The purpose of this study was to isolate and identify antioxidant compounds in watercress. The studies included maceration and reflux extraction with 96% ethanol, partitioning (with n-hexane and ethyl acetate), antioxidant activity determination, and compound determination. Antioxidant activity test by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenger method showed that the watercress ethyl acetate extract had the highest antioxidant activity with IC50 of 67.77 ppm. Fractionation by first column chromatography [SiO2; n-hexane: ethyl acetate (10:1–1:1), CHCl3: MeOH (15:1–1:1)] obtained 5 combined fractions (F1–F5) where the results of the antioxidant activity test of fraction 5 (F5) had a great inhibition value compared to other fractions of 61.04%. Furthermore, F5 fractionated second column chromatography [SiO2; n-hexane: ethyl acetate (1:1), CHCl3: MeOH (5:1)] obtained 2 combined fractions (F5.1 and F5.2). After two purifications using column chromatography and testing for antioxidant activity, fraction 5.2 had the highest activity with an IC50 value of 75.65 ppm. Fraction 5.2 contained phenols and/or flavonoids according to phytochemical screening, while UV-Vis, Fourier transform infrared (FTIR) spectrophotometry and gas chromatography-mass spectrometry (GC-MS) spectroscopic results indicated that, the active ingredient in fraction 5.2 is expected to be 2-methoxy-4-vinylphenol.
- Copyright
- © 2023 The Author(s)
- Open Access
- Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.
Cite this article
TY - CONF AU - Bustanussalam AU - Yatri Hapsari AU - Fauzy Rachman AU - Eris Septiana AU - Partomuan Simanjuntak PY - 2023 DA - 2023/03/01 TI - Identification of Antioxidant Active Compounds from Watercress (Nasturtium officinale R.Br) BT - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022) PB - Atlantis Press SP - 374 EP - 384 SN - 2468-5739 UR - https://doi.org/10.2991/978-94-6463-112-8_35 DO - 10.2991/978-94-6463-112-8_35 ID - 2023 ER -