Antioxidant Activity of Ethanolic Extract of Peel and Seed Melinjo (Gnetum gnemon) Based on Color Variations

DOI

10.2991/978-94-6463-112-8_25How to use a DOI?

Keywords

Gnetum gnemon; endosperm seeds; peel; color variations; antioxidants

Abstract

Melinjo (Gnetum gnemon Linn) is a plant of the genus Gnetacea that has been identified as having various biological activities, including antioxidants. This study aimed to analyze the biological activity of Gnetum gnemon for the determination of total phenolics, anti-free radicals (antioxidants), and the identification of chemical compounds contained in ethanol extract from the peel and inner seeds (endosperm). The total phenol and antioxidant activity of the peel and endosperm of G. gnemon seeds based on color variations have been investigated. The total phenolic content was analyzed by the Folin-Ciocalteu method. Antioxidant activity was analyzed by NO, FRAP, and Trolox as comparison standards which showed antioxidant activity. Identification of compounds in the extract using Thin Layer Chromatography (TLC) and Gas Chromatography-Mass Spectroscopy (GCSM). The results showed that the endosperm extract of the yellow seed G. gnemon had the highest total phenolic content, at 99.96 mg GAE/g sample and the lowest was in the red peel extract at 27.53 mg GAE/g sample. Likewise, the green seed extract had the highest NO scavenging activity of 43.80% and the lowest was the red peel extract which detected no inhibition of antioxidant activity. On the other hand, in the FRAP test, the activity of G. gnemon peel extract had a stronger reducing power than the endosperm seed extract in all color variations. The compound profile in the test extract showed a fairly clear spot of the compound under UV light at 366 nm and the stain appearance. About 10 compounds were detected in the GC-MS spectrum, which had a similarity index equal to or greater than 90%. However, there was no relationship between the maturity level of the melinjo color and the antioxidant activity tested.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Martha Sari
AU  - Siti I. Rahmawati
AU  - Fauzia N. Izzati
AU  - Bustanussalam
AU  - Masteria Y. Putra
PY  - 2023
DA  - 2023/03/01
TI  - Antioxidant Activity of Ethanolic Extract of Peel and Seed Melinjo (Gnetum gnemon) Based on Color Variations
BT  - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
PB  - Atlantis Press
SP  - 255
EP  - 265
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-112-8_25
DO  - 10.2991/978-94-6463-112-8_25
ID  - Sari2023
ER  -