Antibacterial Properties of Bacteria Associated with a Marine Sponge from Thousand Islands, Indonesia

DOI

10.2991/978-94-6463-112-8_5How to use a DOI?

Keywords

marine product; Lithistid sponge; antibiotics; GC-MS; 16S rRNA

Abstract

Marine invertebrates, especially sponges, have been recognized as the source of marine natural products with various bioactivities. The problematic sustainability of the natural product research from sponges drives the research change from the sponge to the associated microbes. In the attempt to find new antibiotic candidates, we screened marine sponges for their bioactivities. A Lithistid sponge showed potential antibiotic activity. The difficulty in obtaining large amounts of Lithistid sponge samples hampered the development of potentially active compounds from this material. Several researches proved that sponge-associated microbial play an important role for producing active compounds. In this study, we focused on sponge-associated bacteria to find novel and potential candidates for antibiotics. In total, we isolated 16 strains of microbes from the Lithistid sponge. A preliminary study of antibacterial activities from the strains M4 SP3 121015 101F showed that a sample has a strong activity against Bacillus subtilis. Partial identification of the bacterium using 16S rRNA Sanger sequencing showed 100% sequence similarity to Staphylococcus equorum. The preliminary identification of substances contained in ethyl acetate extracts using GC-MS showed several substances such as fatty acid, phenol, sterol, and halogenated organic compound. Further chemical analysis of the active fraction using LC-MS/MS showed a putative compound with m/z of 443.07 as the major component and responsible for the bioactivity of the bacterium. The predictions of fragmentation suggested that the compounds were 5-Bromo-4-fluoro-2-methoxyphenyl)(5-iodo-3-thienyl)methanol. This study reported for the first time that the antibacterial activity against Bacillus subtilis showed by the substance produced by marine Staphylococcus equorum isolated from the Lithistid sponge. Nonetheless, further research for detailed characterization of potential compounds is indispensable.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Tutik Murniasih
AU  - Joko T. Wibowo
AU  - Masteria Y. Putra
AU  - Febriana Untari
AU  - Rosinta Handinata
PY  - 2023
DA  - 2023/03/01
TI  - Antibacterial Properties of Bacteria Associated with a Marine Sponge from Thousand Islands, Indonesia
BT  - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
PB  - Atlantis Press
SP  - 38
EP  - 48
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-112-8_5
DO  - 10.2991/978-94-6463-112-8_5
ID  - Murniasih2023
ER  -