The Change of Aquaporin 3 Expression in Colonic Tissues of Different Degrees Diarrhea Model Rats

DOI

10.2991/icmmcce-17.2017.23How to use a DOI?

Keywords

Different degree of diarrhea; Colon. Aquaporin 3

Abstract

Objective: To study the change of AQP3 expression in colonic tissues of different degrees' diarrhea model rats. Methods: SD rats were randomly divided into four groups: control group, mild diarrhea group, moderate diarrhea group and severe diarrhea group. the rat models of diarrhea were established by using different concentrations of sennan intragastric administration for 7d; The water content of feces was measured. Using optical microscopy to observe the pathological and morphological changes in colonic tissues of different degrees' diarrhea model rats; The expression of AQP3 protein in colonic tissues were detected by immunohistochemistry assay and Western blot. The expression of AQP3 mRNA were detected by RT-PCR. Results The water contents of feces were significantly higher in diarrhea model groups (P<0.01), pairwise comparison, the difference were statistically significant (P<0.05); HE staining showed, compared with the control group, there were more sticky secretions in diarrhea groups, glandular cells of mucous membrane laye markedly swollen, we could see a large number of congested capillaries in submucosa, the heavier the degree of diarrhea, the more obvious change; Immunohistochemistry assay and Western blot, RT-PCR results showed that compared with the control group, AQP3 protein and AQP3 mRNA expression in colon were reduced in diarrhea groups(P<0.01), and the heavier the degree of diarrhea, the lower expression quantity (P<0.05).Conclusion AQP3 expression in diarrhea rats colon were decreased, and the heavier the degree of diarrhea, the lower expression quantity.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yiwei Tang
AU  - Wei Zhou
AU  - Jia Chen
AU  - Long Xiang
PY  - 2017/09
DA  - 2017/09
TI  - The Change of Aquaporin 3 Expression in Colonic Tissues of Different Degrees Diarrhea Model Rats
BT  - Proceedings of the 2017 5th International Conference on Mechatronics, Materials, Chemistry and Computer Engineering (ICMMCCE 2017)
PB  - Atlantis Press
SP  - 111
EP  - 115
SN  - 2352-5401
UR  - https://doi.org/10.2991/icmmcce-17.2017.23
DO  - 10.2991/icmmcce-17.2017.23
ID  - Tang2017/09
ER  -