Proceedings of the Third International Conference on Sustainable Innovation 2019 – Health Science and Nursing (IcoSIHSN 2019)

Chemopreventive Activity of Roselle’s Hexane Fraction against Breast Cancer by In-Vitro and In-Silico Study

Authors
Nabila Kaulika, Rifki Febriansah
Corresponding Author
Rifki Febriansah
Available Online October 2019.
DOI
https://doi.org/10.2991/icosihsn-19.2019.16How to use a DOI?
Keywords
Hibiscus sabdariffa; cytotoxic MTT, antioxidant DPPH, molecular docking, T47D breast cancer
Abstract
Breast cancer is ranked the highest percentage of the new cases and deaths in women around the world. Cancer treatments that currently exist cause side effects that are detrimental to patients triggering the development of cancer treatment that comes from nature or herbal plants. Roselle (Hibiscus sabdariffa L.) is a plant that is known to have flavonoids which are potential as anticancer agent. This study was conducted to analyze the roselle's hexane fraction as a chemopreventive agent with an antioxidant test using the DPPH method, in-vitro cytotoxic test of T47D cancer cells line by MTT method, and in-silico test using Vina molecular docking method. The results of the antioxidant test showed the IC50 value of 4259 µg/mL. Cytotoxic activity of the fraction on T47D breast cancer cells showed the IC50 values of 331.86 µg/ml. The molecular docking results obtained a docking score that shows the binding energy of cyanidin 3-O-glucoside to EGFR and HER-2 proteins of -8.2 kcal/mol and 8.1 kcal/mol, respectively. Based on these results, it can be concluded that roselle hexane fraction has a potential as a chemopreventive agent based on its molecular docking and cytotoxic activity against T47D breast cancer cells
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Proceedings
Third International Conference on Sustainable Innovation 2019 – Health Science and Nursing (IcoSIHSN 2019)
Part of series
Advances in Health Sciences Research
Publication Date
October 2019
ISBN
978-94-6252-814-7
ISSN
2468-5739
DOI
https://doi.org/10.2991/icosihsn-19.2019.16How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Nabila Kaulika
AU  - Rifki Febriansah
PY  - 2019/10
DA  - 2019/10
TI  - Chemopreventive Activity of Roselle’s Hexane Fraction against Breast Cancer by In-Vitro and In-Silico Study
BT  - Third International Conference on Sustainable Innovation 2019 – Health Science and Nursing (IcoSIHSN 2019)
PB  - Atlantis Press
SP  - 66
EP  - 71
SN  - 2468-5739
UR  - https://doi.org/10.2991/icosihsn-19.2019.16
DO  - https://doi.org/10.2991/icosihsn-19.2019.16
ID  - Kaulika2019/10
ER  -