Determination of Methampyrone Levels Using Thin Layer Chromatography and UV Spectrophotometry Method in Gout Herbal Medicine
- DOI
- 10.2991/ahsr.k.210115.094How to use a DOI?
- Keywords
- MTP, TLC, Spectrophotometry UV, Gout Herbal Medicine
- Abstract
Based on data from the National Food and Drug Administration, there are 46 withdrawals of herbal products on the market because they contain hazardous chemicals such as MTP, phenylbutazone, dexamethasone, allopurinol, CTM, sildenafil citrate, tadalafil, and paracetamol. MTP is one of the analgesic-antipyretic drugs. However, MTP is widely known to be mixed into rheumatic herbs and gout. The use of high doses of MTP can cause side effects namely, stomach bleeding, palpitations, heart damage, and others. The purpose of this study was to determine the amount of MTP in a gout herbal sample. The sample used in this study was a sample of herbal gout powder sold in herbal stalls in the city of Tangerang. Samples are taken by random sampling method. The MTP qualitative analysis method uses thin layer chromatography. The stationary phase used was silica gel GF 254 while the mobile phase used consisted of methanol : ammonia (100: 1.5). The quantitative analysis method used the UV Spectrophotometry method with a maximum wavelength of 258.5 nm. Qualitative analysis showed that the Rf value of the herbal gout powder samples approached the Rf value of MTP and it was proven by the presence of purple spots under UV light. Furthermore, quantitative analysis showed that the levels of MTP contained in the herbal gout powder sample were 72.59±1.12 sample A, 65.34±1.13 sample B, 11.45±0.98 sample C, and 6.70±0.64 sample D, respectively. The results showed that the herbal gout powder samples were positive contains MTP.
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Nita Rusdiana AU - Diana Kartika Wulansari AU - Diana Sylvia PY - 2021 DA - 2021/01/16 TI - Determination of Methampyrone Levels Using Thin Layer Chromatography and UV Spectrophotometry Method in Gout Herbal Medicine BT - Proceedings of the 4th International Conference on Sustainable Innovation 2020–Health Science and Nursing (ICoSIHSN 2020) PB - Atlantis Press SP - 479 EP - 483 SN - 2468-5739 UR - https://doi.org/10.2991/ahsr.k.210115.094 DO - 10.2991/ahsr.k.210115.094 ID - Rusdiana2021 ER -