Chemopreventive Activity of Clinacanthus nutans L. Ethyl Acetate Fraction on Breast Cancer Cells Line

DOI

10.2991/ahsr.k.210115.102How to use a DOI?

Keywords

Clinacanthus nutans, molecular docking, cytotoxic, T47D, Vero

Abstract

Cancer is a non-communicable disease, the second largest cause of death in the world. In Indonesia, breast cancer is the most common type of cancer suffered by women with 22,692 deaths case. However, chemotherapy drugs work non-selectively as it they are against both cancer cells and normal cells, which cause severe side effects. Further, a chemo-preventive agent from nature needs to be developed. This study aims to determine the chemo-preventive activity of Clinacanthus nutans L. Ethyl Acetate fraction by in vitro and in silico study. By the procedure, the plant powders were macerated with ethanol 70% followed by fractionation using ethyl acetate, and thin Layer Chromatography was used to identify active compounds in it. Molecular docking was carried out using Autodock Vina for in silico study to visualize affinity between compounds and EGFR and HER-2 protein. The antioxidant test applied the DPPH method, where quercetin became the standard. The cytotoxic activity was evaluated using the MTT Assay. The results were expressed as inhibitory concentration (IC50), and Selectivity Index (SI) was determined by calculating the ratio of the IC50 on Vero cells line and T47D cells line. The results showed that EtOAc fraction contained flavonoids. Based on molecular docking result, vitexin has stronger binding affinity with HER-2 protein than EGFR (-9.3 kcal/mol) compared to doxorubicin. The Clinacanthus nutans L. Ethyl Acetate fraction had a weak antioxidant ability with IC50 of 576,904 µg/mL while quercetin (positive control) resulted 6,874 µg/mL. Single cytotoxic assay on Vero and T47D cell line resulted the IC50 values of 1452,835 μg/mL and 340,122 μg/mL, respectively, with SI value of 4,271. The results concluded that Clinacanthus nutans L. Ethyl Acetate Fraction is less toxic on T47D breast cancer cells line but selective on Vero normal cells line.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Rifki Febriansah
AU  - Isna Aura Dewayanti
PY  - 2021
DA  - 2021/01/16
TI  - Chemopreventive Activity of Clinacanthus nutans L. Ethyl Acetate Fraction on Breast Cancer Cells Line
BT  - Proceedings of the 4th International Conference on Sustainable Innovation 2020–Health Science and Nursing (ICoSIHSN 2020)
PB  - Atlantis Press
SP  - 520
EP  - 527
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.210115.102
DO  - 10.2991/ahsr.k.210115.102
ID  - Febriansah2021
ER  -