The Effect of Dioscorea alata Extract on the Early Development of Zebrafish Embryo (Danio rerio) and Rasbora lateristriata

DOI

10.2991/assehr.k.210312.096How to use a DOI?

Keywords

Millennial generation, Creative industry, Poverty

Abstract

Dioscorea alata is reported to have many benefits and potentials, one of which is anti-diabetes since it contains Dioscorin and Glucomannan. D. alata used in this research is purple cultivar since it has higher antioxidant content than the other cultivar. This research will examine D. alata extract’s effects on the early development of zebrafish (Danio rerio) and yellow rasbora (Rasbora lateristriata) embryos. The D. rerio and R. lateristriata embryos treatment was carried out on 3 hours post-fertilization (hpf) embryos aged. The embryos were exposed to D. alata, with 100mg/100ml, 500mg/100ml, and 1000mg/100ml. Biocompatibility assessment was carried out by measuring the hatching rate, survival rate, morphological changes, and heartbeat rate, respectively. The hatching rate was determined as the number of embryos that hatched at observation time. The survival rate was determined by the number of survive embryos at 12, 24, 36, 48, 60, 72 and 84 hpf, respectively. Moreover, the morphological change was observed on the shape of the embryo’s heart and yolk and the tail-yolk detachment as an indicator of a normal body wall occurrence, respectively. Moreover, the physiological performance was decided base on the heartbeat rate performance of the embryos. Statistical analysis was performed by using the SPSS 21 application. One-way ANOVA was used to assess the significance of the effect of treatment on outcomes. The result showed a significant effect of D. alata on the hatching rate and survival rate, but it does not effect the embryo morphology and the heart rate in all treatments to Rasbora lateristriata and Danio rerio.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Anita Raharjeng
AU  - Bambang Retnoaji
PY  - 2021
DA  - 2021/03/13
TI  - The Effect of Dioscorea alata Extract on the Early Development of Zebrafish Embryo (Danio rerio) and Rasbora lateristriata
BT  - Proceedings of the First International Conference on Science, Technology, Engineering and Industrial Revolution (ICSTEIR 2020)
PB  - Atlantis Press
SP  - 601
EP  - 611
SN  - 2352-5398
UR  - https://doi.org/10.2991/assehr.k.210312.096
DO  - 10.2991/assehr.k.210312.096
ID  - Raharjeng2021
ER  -