Proceedings of the 11th International Dentistry Scientific Meeting (IDSM 2017)

In Vitro Chitosan Hydrogel Based Metronidazole Cytotoxicity Test on 3t3 Fibroblast Viability

Authors
Hilma Rasni, Irma Ervina, Syafruddin Ilyas
Corresponding Author
Hilma Rasni
Available Online May 2018.
DOI
https://doi.org/10.2991/idsm-17.2018.44How to use a DOI?
Keywords
metronidazole, viability, cytotoxicity, fibroblast, chitosan.
Abstract
The objective of this study is to determine the in vitro cytotoxicity effect of chitosan hydrogel-based metronidazole on fibroblast. Chitosan hydrogel-based metronidazole(0.12%, 0.15%, 0.25%) and chitosan without metronidazole are made by dissolving lactic acid to reach a gel consistency, and then adding powdered metronidazole according to the concentration. 3T3 fibroblast cells were cultured in well mikroplate containing RPMI-1640 media inside an incubator. The cell viability tests were carried out using theMTT assay method and repeated five times. The colour degradation was read with a microplate reader. The mean viability of fibroblasts mixed with chitosan hydrogel-based metronidazole at concentration of 0.12%, 0.15%, and 0.25%, and chitosan without metronidazole were 96%, 93%, 91% and 98.8% respectively. ANOVA statistical test results for the 0.12% concentration group, where p < 0.05 represents the cell’s viability, significantly differs from the results for the 0.15% and 0.25% concentration groups. Chitosan hydrogel-based metronidazole shows non toxic effects on fibroblast cells.
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This is an open access article distributed under the CC BY-NC license.

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Proceedings
11th International Dentistry Scientific Meeting (IDSM 2017)
Part of series
Advances in Health Sciences Research
Publication Date
May 2018
ISBN
978-94-6252-513-9
ISSN
2468-5739
DOI
https://doi.org/10.2991/idsm-17.2018.44How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Hilma Rasni
AU  - Irma Ervina
AU  - Syafruddin Ilyas
PY  - 2018/05
DA  - 2018/05
TI  - In Vitro Chitosan Hydrogel Based Metronidazole Cytotoxicity Test on 3t3 Fibroblast Viability
BT  - 11th International Dentistry Scientific Meeting (IDSM 2017)
PB  - Atlantis Press
SN  - 2468-5739
UR  - https://doi.org/10.2991/idsm-17.2018.44
DO  - https://doi.org/10.2991/idsm-17.2018.44
ID  - Rasni2018/05
ER  -